Introduction: Gratitude is widely contemplated in philosophy and theology, although its dissemination in psychology and health sciences is scarce. It is defined as the cognitive and affective state of appreciation in a person favored by a benefactor’s contributions. The Gratitude Questionnaire–Six Item Form is one of the most used scales for measuring this construct in several sociocultural contexts. Objectives: The aims were to determine the factorial, convergent, and discriminant validity and to identify its reliability and invariance according to gender, age, and residence. Method:Instrumental research was conducted with 350 Venezuelans. Results: The six–item model exhibited a poor fit, leading to the formulation of five alternative models. The unifactorial structure of six items with correlated residuals showed a good fit, although the average variance extracted was low. Cronbach’s Alpha reported an acceptable value, yet McDonald’s Omega was relatively below the recommended cut–off. The questionnaire exhibited correlations with external variables and confirmed full and partial scalar invariance. Conclusions: The properties of the GQ–6 make it suitable for its use in the Venezuelan context; however, its limitations should be reviewed to improve it. It is concluded that the questionnaire can be used to assess gratitude as a protective factor of mental health and as an element to promote well–being and meaningful life