Lignocellulosic materials can be transformed into value-added products such as biofuels (bioethanol, biobutanol), organic acids (lactic, succinic acid), acetone, etc. For its transformation into these products, a first pretreatment step is required to disrupt the lignocellulosic matrix, followed by a hydrolysis step to obtain fermentable sugars. The main aim of this work was to evaluate the effect of pH, solid content, and enzyme dosage on the enzymatic hydrolysis of steam-exploded Eucalyptus grandis wood, previously impregnated with sulfuric acid at 0.5% (w/w). The studied factors were solid content (14.6-25.4) % (w/w), enzyme dosage (15-25) FPU/gglucan and pH (4.6-6.3). Results showed that the conditions which maximized both glucose concentration and hydrolysis efficiency corresponded to pH 6, 24% (w/w) solid content, and 25 FPU/gglucan enzyme dosage