Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve
Editor(es): Wiche, G.
Resumen:
Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal and regenerating peripheral nerves. Previously, we have shown that RNAs transfer is dependent on F-actin cytoskeleton and Myosin Va. Here, we explored the contribution of microtubules to newly synthesized RNAs transport from Schwann cell nuclei up to nodal microvilli in sciatic nerves. Results using immunohistochemistry and quantitative confocal FRET analysis indicate that Schwann cell-derived RNAs co-localize with microtubules in Schwann cell cytoplasm. Additionally, transport of Schwann cell-derived RNAs is nocodazole and colchicine sensitive demonstrating its dependence on microtubule network integrity. Moreover, mRNAs codifying neuron-specific proteins are among Schwann cell newly synthesized RNAs population, and some of them are associated with KIF1B and KIF5B microtubules-based motors.
| 2020 | |
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RNA Axons Microtubules Nerve Regeneration Schwann Cells Sciatic Nerve |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/31776 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución (CC - By 4.0) |
| _version_ | 1807522789880496128 |
|---|---|
| author | Canclini, Lucía |
| author2 | Farías, Joaquina Di Paolo, Andrés Sotelo Silveira, José Roberto Folle Ungo, Gustavo A. Kun González, Alejandra E. Sotelo Sosa, José Roberto |
| author2_role | author author author author author author |
| author_facet | Canclini, Lucía Farías, Joaquina Di Paolo, Andrés Sotelo Silveira, José Roberto Folle Ungo, Gustavo A. Kun González, Alejandra E. Sotelo Sosa, José Roberto |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Canclini Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología Farías Joaquina, IIBCE Di Paolo Forné Andrés Pablo, IIBCE Sotelo Silveira José Roberto, IIBCE Folle Ungo Gustavo A., IIBCE Kun González Alejandra E., Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología Sotelo Sosa José Roberto, IIBCE |
| dc.creator.editor.none.fl_str_mv | Wiche, G. |
| dc.creator.none.fl_str_mv | Canclini, Lucía Farías, Joaquina Di Paolo, Andrés Sotelo Silveira, José Roberto Folle Ungo, Gustavo A. Kun González, Alejandra E. Sotelo Sosa, José Roberto |
| dc.date.accessioned.none.fl_str_mv | 2022-06-01T13:37:37Z |
| dc.date.available.none.fl_str_mv | 2022-06-01T13:37:37Z |
| dc.date.issued.none.fl_str_mv | 2020 |
| dc.description.abstract.none.fl_txt_mv | Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal and regenerating peripheral nerves. Previously, we have shown that RNAs transfer is dependent on F-actin cytoskeleton and Myosin Va. Here, we explored the contribution of microtubules to newly synthesized RNAs transport from Schwann cell nuclei up to nodal microvilli in sciatic nerves. Results using immunohistochemistry and quantitative confocal FRET analysis indicate that Schwann cell-derived RNAs co-localize with microtubules in Schwann cell cytoplasm. Additionally, transport of Schwann cell-derived RNAs is nocodazole and colchicine sensitive demonstrating its dependence on microtubule network integrity. Moreover, mRNAs codifying neuron-specific proteins are among Schwann cell newly synthesized RNAs population, and some of them are associated with KIF1B and KIF5B microtubules-based motors. |
| dc.format.extent.es.fl_str_mv | 18 h. |
| dc.format.mimetype.es.fl_str_mv | application/pdf |
| dc.identifier.citation.es.fl_str_mv | Canclini, L, Farías, J, Di Paolo, A, [y otros] "Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve". PLoS ONE. [en línea] 2020, 15(5): e0233651. 18 h. DOI: 10.1371/journal.pone.0233651 |
| dc.identifier.doi.none.fl_str_mv | 10.1371/journal.pone.0233651 |
| dc.identifier.issn.none.fl_str_mv | 1932-6203 |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/31776 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.publisher.en.fl_str_mv | Public Library of Science |
| dc.relation.ispartof.es.fl_str_mv | PLoS ONE, 2020, 15(5): e0233651 |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.en.fl_str_mv | RNA Axons Microtubules Nerve Regeneration Schwann Cells Sciatic Nerve |
| dc.title.none.fl_str_mv | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal and regenerating peripheral nerves. Previously, we have shown that RNAs transfer is dependent on F-actin cytoskeleton and Myosin Va. Here, we explored the contribution of microtubules to newly synthesized RNAs transport from Schwann cell nuclei up to nodal microvilli in sciatic nerves. Results using immunohistochemistry and quantitative confocal FRET analysis indicate that Schwann cell-derived RNAs co-localize with microtubules in Schwann cell cytoplasm. Additionally, transport of Schwann cell-derived RNAs is nocodazole and colchicine sensitive demonstrating its dependence on microtubule network integrity. Moreover, mRNAs codifying neuron-specific proteins are among Schwann cell newly synthesized RNAs population, and some of them are associated with KIF1B and KIF5B microtubules-based motors. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_c7397795865e7ed99bc7eebc1833c6f0 |
| identifier_str_mv | Canclini, L, Farías, J, Di Paolo, A, [y otros] "Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve". PLoS ONE. [en línea] 2020, 15(5): e0233651. 18 h. DOI: 10.1371/journal.pone.0233651 1932-6203 10.1371/journal.pone.0233651 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/31776 |
| publishDate | 2020 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | mabel.seroubian@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Canclini Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de BiologíaFarías Joaquina, IIBCEDi Paolo Forné Andrés Pablo, IIBCESotelo Silveira José Roberto, IIBCEFolle Ungo Gustavo A., IIBCEKun González Alejandra E., Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de BiologíaSotelo Sosa José Roberto, IIBCE2022-06-01T13:37:37Z2022-06-01T13:37:37Z2020Canclini, L, Farías, J, Di Paolo, A, [y otros] "Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve". PLoS ONE. [en línea] 2020, 15(5): e0233651. 18 h. DOI: 10.1371/journal.pone.02336511932-6203https://hdl.handle.net/20.500.12008/3177610.1371/journal.pone.0233651Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal and regenerating peripheral nerves. Previously, we have shown that RNAs transfer is dependent on F-actin cytoskeleton and Myosin Va. Here, we explored the contribution of microtubules to newly synthesized RNAs transport from Schwann cell nuclei up to nodal microvilli in sciatic nerves. Results using immunohistochemistry and quantitative confocal FRET analysis indicate that Schwann cell-derived RNAs co-localize with microtubules in Schwann cell cytoplasm. Additionally, transport of Schwann cell-derived RNAs is nocodazole and colchicine sensitive demonstrating its dependence on microtubule network integrity. Moreover, mRNAs codifying neuron-specific proteins are among Schwann cell newly synthesized RNAs population, and some of them are associated with KIF1B and KIF5B microtubules-based motors.Submitted by Verdun Juan Pablo (jverdun@fcien.edu.uy) on 2022-05-30T22:50:29Z No. of bitstreams: 2 license_rdf: 19875 bytes, checksum: 9fdbed07f52437945402c4e70fa4773e (MD5) 10.1371journal.pone.0233651.pdf: 2804325 bytes, checksum: 2614e0f9149bbacfe8ba8f14ad1011ae (MD5)Approved for entry into archive by Faget Cecilia (lfaget@fcien.edu.uy) on 2022-06-01T12:44:00Z (GMT) No. of bitstreams: 2 license_rdf: 19875 bytes, checksum: 9fdbed07f52437945402c4e70fa4773e (MD5) 10.1371journal.pone.0233651.pdf: 2804325 bytes, checksum: 2614e0f9149bbacfe8ba8f14ad1011ae (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2022-06-01T13:37:37Z (GMT). No. of bitstreams: 2 license_rdf: 19875 bytes, checksum: 9fdbed07f52437945402c4e70fa4773e (MD5) 10.1371journal.pone.0233651.pdf: 2804325 bytes, checksum: 2614e0f9149bbacfe8ba8f14ad1011ae (MD5) Previous issue date: 202018 h.application/pdfenengPublic Library of SciencePLoS ONE, 2020, 15(5): e0233651Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)RNAAxonsMicrotubulesNerve RegenerationSchwann CellsSciatic NerveAssociation of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerveArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaCanclini, LucíaFarías, JoaquinaDi Paolo, AndrésSotelo Silveira, José RobertoFolle Ungo, Gustavo A.Kun González, Alejandra E.Sotelo Sosa, José RobertoWiche, G.LICENSElicense.txtlicense.txttext/plain; charset=utf-84267http://localhost:8080/xmlui/bitstream/20.500.12008/31776/5/license.txt6429389a7df7277b72b7924fdc7d47a9MD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-844http://localhost:8080/xmlui/bitstream/20.500.12008/31776/2/license_urla0ebbeafb9d2ec7cbb19d7137ebc392cMD52license_textlicense_texttext/html; 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- Universidad de la Repúblicafalse |
| spellingShingle | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve Canclini, Lucía RNA Axons Microtubules Nerve Regeneration Schwann Cells Sciatic Nerve |
| status_str | publishedVersion |
| title | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_full | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_fullStr | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_full_unstemmed | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_short | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| title_sort | Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve |
| topic | RNA Axons Microtubules Nerve Regeneration Schwann Cells Sciatic Nerve |
| url | https://hdl.handle.net/20.500.12008/31776 |
