Association of microtubules and axonal RNA transferred from myelinating Schwann cells in rat sciatic nerve
- Autor(es):
- Canclini, Lucía ; Farías, Joaquina ; Di Paolo, Andrés ; Sotelo Silveira, José Roberto ; Folle Ungo, Gustavo A. ; Kun González, Alejandra E. ; Sotelo Sosa, José Roberto ; Wiche, G.
- Tipo:
- Artículo
- Versión:
- Publicado
- Resumen:
-
Transference of RNAs and ribosomes from Schwann cell-to-axon was demonstrated in normal and regenerating peripheral nerves. Previously, we have shown that RNAs transfer is dependent on F-actin cytoskeleton and Myosin Va. Here, we explored the contribution of microtubules to newly synthesized RNAs transport from Schwann cell nuclei up to nodal microvilli in sciatic nerves. Results using immunohistochemistry and quantitative confocal FRET analysis indicate that Schwann cell-derived RNAs co-localize with microtubules in Schwann cell cytoplasm. Additionally, transport of Schwann cell-derived RNAs is nocodazole and colchicine sensitive demonstrating its dependence on microtubule network integrity. Moreover, mRNAs codifying neuron-specific proteins are among Schwann cell newly synthesized RNAs population, and some of them are associated with KIF1B and KIF5B microtubules-based motors.
- Año:
- 2020
- Idioma:
- Inglés
- Temas:
- RNA
Axons
Microtubules
Nerve Regeneration
Schwann Cells
Sciatic Nerve
- Institución:
- Universidad de la República
- Repositorio:
- COLIBRI
- Enlace(s):
- https://hdl.handle.net/20.500.12008/31776
- Nivel de acceso:
- Acceso abierto