Characterization and application of recombinant Bovine Leukemia Virus Env protein
Resumen:
The Bovine Leukemia Virus (BLV) Envelope (Env) glycoprotein complex is instrumental in viral infectivity and shapes the host’s immune response. This study presents the production and characterization of a soluble furin‑mutated BLV Env ectodomain (sBLV‑EnvFm) expressed in a stable S2 insect cell line. We purified a 63 kDa soluble protein, corresponding to the monomeric sBLV‑EnvFm, which predominantly presented oligomannose and paucimannose N-glycans, with a high content of core fucose structures. Our results demonstrate that our recombinant protein can be recognized from specific antibodies in BLV infected cattle, suggesting its potential as a powerful diagnostic tool. Moreover, the robust humoral immune response it elicited in mice shows its potential contribution to the development of subunit‑based vaccines ag
| 2024 | |
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ANII: POS_NAC_2010_1_2249 ANII: POS_NAC_2015_1_109471 |
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Biochemistry Immunology Bovine Leukemia Virus Glycoprotein Diagnostic |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/51033 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución (CC - By 4.0) |
