In this chapter, a case study is presented on the evaluation of acoustic performance of a tree barrier. It is a eucalyptus barrier that was planted as a visual barrier to block an industrial plant. First, the depletion law of sound pressure levels (SPL) of the source was analyzed; a linear divergence was found. A calculation scheme similar to that of ISO 9613-2 was applied. When comparing the SPL measured at a specific receiver with the results of propagating the SPL from the source without considering the existence of the barrier, an extra attenuation of 12 dB appeared, reinforcing the idea that the plantation behaves as an acoustic barrier. Four different calculations were used to obtain its insertion loss (IL), including general equations and expressions developed for green barriers. The best fit was obtained using equations for solid barriers, although it was not the expected result. This finding could be explained by the great distance between the source and the receiver. It opens the possibility of successfully using IL prediction equations for solid acoustic barriers (both thin and thick) to estimate the acoustic performance of green barriers, at least under conditions similar to those of this case study.