Global annual losses of USD billions are reported in milk production due to mastitis. Antibiotics are used in dairy farms for the treatment and prevention of diseases in dairy cattle. There are national and international regulations that prohibit the processing of milk with antibiotics, so it is important to study alternatives to final disposal. Veterinary drug residues are considered a danger and potential risk for public health, dairy industrialization processes and the environment. There are different techniques for the treatment of milk contaminated with antibiotics. Some of these techniques are very expensive and have low yields, others use very toxic reagents and generate toxic by-products for the environment. The use of enzymes, specially laccases, in biodegradation processes have many advantages as they are safer, less disruptive, less expensive, require lower energy employment, considered as a green catalysis processes. Enzyme immobilization is an enabling technology for application of enzyme in industrial biocatalytic processes. Particularly, cross linked enzyme aggregates (CLEAs) are facile and cost-effective to prepare; the general procedure consists of enzyme precipitation and subsequent cross-linking with a bifunctional agent. With this immobilization strategy we are capable to generate diverse biocatalysts. These can be reused in bioprocesses and allow continuous use, thus simplifying processes and reducing costs.