Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry
Resumen:
Oct4 is involved in regulation of pluripotency during normal development and is down-regulated during formation of postnatal reservoir of germ cells. We propose thatOct4/GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, could be used as a mammalian model to study the effects of environmental estrogens on the development of male germ cells. Oct4/GFP maturation profile was assessed during postnatal days -PND- 3, 5, 7, 10, 14 and 80, using flow cytometry. Then, we exposed pregnant mothers to 17a-ethinylestradiol (EE2) from day post coitum (dpc) 5 to PND7. Percentage of Oct4/GFP-expressing cells and levels of expression of Oct4/GPF were increased in PND7 after EE2 exposure. These observations were confirmed by analysis of GFP and endogenous Oct4 protein in the seminiferous tubules and by a reduction in epididymal sperm count in adult mice. We introduced Oct4/ GFP mouse together with flow cytometry as a tool to evaluate changes in male germ cells development.
| 2015 | |
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Agencia Nacional de Investigación e Innovación FONCyT (Fondo para la InvestigaciónCientífica y Tecnológica de la Agencia Nacional de PromociónCientífica y Tecnológica, ANPCyT, Argentina) FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 CONICET (becas doctoral y postdoctoral) |
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Estrogens Flow cytometry GFP Male germ cells Maturation Ciencias Médicas y de la Salud |
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| Inglés | |
| Institut Pasteur de Montevideo | |
| IPMON en REDI | |
| http://hdl.handle.net/20.500.12381/201 | |
| Acceso abierto | |
| Reconocimiento-NoComercial-SinObraDerivada. (CC BY-NC-ND) |
| _version_ | 1808165740477415424 |
|---|---|
| author | Porro, Valentina |
| author2 | Pagotto, Romina Harreguy, María Belén Ramírez, Sofía Crispo, Martina Santamaría, Clarisa Luque, Enrique H. Rodríguez, Horacio A. Bollati-Fogolín, Mariela |
| author2_role | author author author author author author author author |
| author_facet | Porro, Valentina Pagotto, Romina Harreguy, María Belén Ramírez, Sofía Crispo, Martina Santamaría, Clarisa Luque, Enrique H. Rodríguez, Horacio A. Bollati-Fogolín, Mariela |
| author_role | author |
| bitstream.checksum.fl_str_mv | 2d97768b1a25a7df5a347bb58fd2d77f 02934e0f17afd434fbc587d10052c804 |
| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 |
| bitstream.url.fl_str_mv | https://redi.anii.org.uy/jspui/bitstream/20.500.12381/201/2/license.txt https://redi.anii.org.uy/jspui/bitstream/20.500.12381/201/1/Porro%20et%20al%202015.pdf |
| collection | IPMON en REDI |
| dc.creator.none.fl_str_mv | Porro, Valentina Pagotto, Romina Harreguy, María Belén Ramírez, Sofía Crispo, Martina Santamaría, Clarisa Luque, Enrique H. Rodríguez, Horacio A. Bollati-Fogolín, Mariela |
| dc.date.accessioned.none.fl_str_mv | 2019-11-05T20:47:06Z |
| dc.date.available.none.fl_str_mv | 2019-11-05T20:47:06Z |
| dc.date.issued.none.fl_str_mv | 2015 |
| dc.description.abstract.none.fl_txt_mv | Oct4 is involved in regulation of pluripotency during normal development and is down-regulated during formation of postnatal reservoir of germ cells. We propose thatOct4/GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, could be used as a mammalian model to study the effects of environmental estrogens on the development of male germ cells. Oct4/GFP maturation profile was assessed during postnatal days -PND- 3, 5, 7, 10, 14 and 80, using flow cytometry. Then, we exposed pregnant mothers to 17a-ethinylestradiol (EE2) from day post coitum (dpc) 5 to PND7. Percentage of Oct4/GFP-expressing cells and levels of expression of Oct4/GPF were increased in PND7 after EE2 exposure. These observations were confirmed by analysis of GFP and endogenous Oct4 protein in the seminiferous tubules and by a reduction in epididymal sperm count in adult mice. We introduced Oct4/ GFP mouse together with flow cytometry as a tool to evaluate changes in male germ cells development. |
| dc.description.sponsorship.none.fl_txt_mv | Agencia Nacional de Investigación e Innovación FONCyT (Fondo para la InvestigaciónCientífica y Tecnológica de la Agencia Nacional de PromociónCientífica y Tecnológica, ANPCyT, Argentina) FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11 CONICET (becas doctoral y postdoctoral) |
| dc.format.extent.es.fl_str_mv | 9 p. |
| dc.identifier.anii.es.fl_str_mv | FMV_2_2011_1_6046 |
| dc.identifier.doi.none.fl_str_mv | 10.1016/j.jsbmb.2015.06.006 |
| dc.identifier.uri.none.fl_str_mv | http://hdl.handle.net/20.500.12381/201 |
| dc.language.iso.none.fl_str_mv | eng |
| dc.publisher.es.fl_str_mv | Elsevier |
| dc.rights.es.fl_str_mv | Acceso abierto |
| dc.rights.license.none.fl_str_mv | Reconocimiento-NoComercial-SinObraDerivada. (CC BY-NC-ND) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.es.fl_str_mv | Journal of Steroid Biochemistry and Molecular Biology. 2015; 154: 53-61 |
| dc.source.none.fl_str_mv | reponame:IPMON en REDI instname:Institut Pasteur de Montevideo instacron:Institut Pasteur de Montevideo |
| dc.subject.anii.es.fl_str_mv | Ciencias Médicas y de la Salud |
| dc.subject.es.fl_str_mv | Estrogens Flow cytometry GFP Male germ cells Maturation |
| dc.title.none.fl_str_mv | Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.es.fl_str_mv | Publicado |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Oct4 is involved in regulation of pluripotency during normal development and is down-regulated during formation of postnatal reservoir of germ cells. We propose thatOct4/GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, could be used as a mammalian model to study the effects of environmental estrogens on the development of male germ cells. Oct4/GFP maturation profile was assessed during postnatal days -PND- 3, 5, 7, 10, 14 and 80, using flow cytometry. Then, we exposed pregnant mothers to 17a-ethinylestradiol (EE2) from day post coitum (dpc) 5 to PND7. Percentage of Oct4/GFP-expressing cells and levels of expression of Oct4/GPF were increased in PND7 after EE2 exposure. These observations were confirmed by analysis of GFP and endogenous Oct4 protein in the seminiferous tubules and by a reduction in epididymal sperm count in adult mice. We introduced Oct4/ GFP mouse together with flow cytometry as a tool to evaluate changes in male germ cells development. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | IPMON_0ecf05300239aaf7119733fbdfc141ee |
| identifier_str_mv | FMV_2_2011_1_6046 10.1016/j.jsbmb.2015.06.006 |
| instacron_str | Institut Pasteur de Montevideo |
| institution | Institut Pasteur de Montevideo |
| instname_str | Institut Pasteur de Montevideo |
| language | eng |
| network_acronym_str | IPMON |
| network_name_str | IPMON en REDI |
| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/201 |
| publishDate | 2015 |
| reponame_str | IPMON en REDI |
| repository.mail.fl_str_mv | msarroca@pasteur.edu.uy |
| repository.name.fl_str_mv | IPMON en REDI - Institut Pasteur de Montevideo |
| repository_id_str | 9421_2 |
| rights_invalid_str_mv | Reconocimiento-NoComercial-SinObraDerivada. (CC BY-NC-ND) Acceso abierto |
| spelling | Reconocimiento-NoComercial-SinObraDerivada. (CC BY-NC-ND)Acceso abiertoinfo:eu-repo/semantics/openAccess2019-11-05T20:47:06Z2019-11-05T20:47:06Z2015http://hdl.handle.net/20.500.12381/201FMV_2_2011_1_604610.1016/j.jsbmb.2015.06.006Oct4 is involved in regulation of pluripotency during normal development and is down-regulated during formation of postnatal reservoir of germ cells. We propose thatOct4/GFP transgenic mouse, which mimics the endogenous expression pattern of Oct4, could be used as a mammalian model to study the effects of environmental estrogens on the development of male germ cells. Oct4/GFP maturation profile was assessed during postnatal days -PND- 3, 5, 7, 10, 14 and 80, using flow cytometry. Then, we exposed pregnant mothers to 17a-ethinylestradiol (EE2) from day post coitum (dpc) 5 to PND7. Percentage of Oct4/GFP-expressing cells and levels of expression of Oct4/GPF were increased in PND7 after EE2 exposure. These observations were confirmed by analysis of GFP and endogenous Oct4 protein in the seminiferous tubules and by a reduction in epididymal sperm count in adult mice. We introduced Oct4/ GFP mouse together with flow cytometry as a tool to evaluate changes in male germ cells development.Agencia Nacional de Investigación e InnovaciónFONCyT (Fondo para la InvestigaciónCientífica y Tecnológica de la Agencia Nacional de PromociónCientífica y Tecnológica, ANPCyT, Argentina)FOCEM (MERCOSUR Structural Convergence Fund), COF 03/11CONICET (becas doctoral y postdoctoral)9 p.engElsevierJournal of Steroid Biochemistry and Molecular Biology. 2015; 154: 53-61reponame:IPMON en REDIinstname:Institut Pasteur de Montevideoinstacron:Institut Pasteur de MontevideoEstrogensFlow cytometryGFPMale germ cellsMaturationCiencias Médicas y de la SaludCharacterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometryArtículoPublicadoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitut Pasteur de MontevideoPorro, ValentinaPagotto, RominaHarreguy, María BelénRamírez, SofíaCrispo, MartinaSantamaría, ClarisaLuque, Enrique H.Rodríguez, Horacio A.Bollati-Fogolín, MarielaLICENSElicense.txtlicense.txttext/plain; charset=utf-84746https://redi.anii.org.uy/jspui/bitstream/20.500.12381/201/2/license.txt2d97768b1a25a7df5a347bb58fd2d77fMD52ORIGINALPorro et al 2015.pdfPorro et al 2015.pdfapplication/pdf2178693https://redi.anii.org.uy/jspui/bitstream/20.500.12381/201/1/Porro%20et%20al%202015.pdf02934e0f17afd434fbc587d10052c804MD5120.500.12381/2012022-07-26 13:36:37.547oai:redi.anii.org.uy:20.500.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en REDI - Institut Pasteur de Montevideofalse |
| spellingShingle | Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry Porro, Valentina Estrogens Flow cytometry GFP Male germ cells Maturation Ciencias Médicas y de la Salud |
| status_str | publishedVersion |
| title | Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry |
| title_full | Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry |
| title_fullStr | Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry |
| title_full_unstemmed | Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry |
| title_short | Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry |
| title_sort | Characterization of Oct4-GFP transgenic mice as a model to study the effect of environmental estrogens on the maturation of male germ cells by using flow cytometry |
| topic | Estrogens Flow cytometry GFP Male germ cells Maturation Ciencias Médicas y de la Salud |
| url | http://hdl.handle.net/20.500.12381/201 |
