Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding.
Resumen:
ABSTRACT.The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding.
| 2016 | |
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HAPLOID DOUBLE HAPLOID ANTHER CULTURE FLOW CYTOMETRY PAPA SOLANUM COMMERSONII GERMOPLASMA |
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| Inglés | |
| Instituto Nacional de Investigación Agropecuaria | |
| AINFO | |
| http://www.ainfo.inia.uy/consulta/busca?b=pc&id=56407&biblioteca=vazio&busca=56407&qFacets=56407 | |
| Acceso abierto |
| _version_ | 1805580531678576640 |
|---|---|
| author | CASTILLO, A. |
| author2 | GAIERO, P. LÓPEZ CARRO, B. VILARO, F. |
| author2_role | author author author |
| author_facet | CASTILLO, A. GAIERO, P. LÓPEZ CARRO, B. VILARO, F. |
| author_role | author |
| bitstream.checksum.fl_str_mv | d703f843820ce52fcd137eafd4e6cac8 |
| bitstream.checksumAlgorithm.fl_str_mv | MD5 |
| bitstream.url.fl_str_mv | https://redi.anii.org.uy/jspui/bitstream/20.500.12381/2689/1/sword-2022-12-16T17%3a51%3a01.original.xml |
| collection | AINFO |
| dc.creator.none.fl_str_mv | CASTILLO, A. GAIERO, P. LÓPEZ CARRO, B. VILARO, F. |
| dc.date.accessioned.none.fl_str_mv | 2022-12-16T20:51:01Z |
| dc.date.available.none.fl_str_mv | 2022-12-16T20:51:01Z |
| dc.date.issued.none.fl_str_mv | 2016 |
| dc.date.updated.none.fl_str_mv | 2022-12-16T20:51:01Z |
| dc.description.abstract.none.fl_txt_mv | ABSTRACT.The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. |
| dc.identifier.none.fl_str_mv | http://www.ainfo.inia.uy/consulta/busca?b=pc&id=56407&biblioteca=vazio&busca=56407&qFacets=56407 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.rights.es.fl_str_mv | Acceso abierto |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:AINFO instname:Instituto Nacional de Investigación Agropecuaria instacron:Instituto Nacional de Investigación Agropecuaria |
| dc.subject.none.fl_str_mv | HAPLOID DOUBLE HAPLOID ANTHER CULTURE FLOW CYTOMETRY PAPA SOLANUM COMMERSONII GERMOPLASMA |
| dc.title.none.fl_str_mv | Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
| dc.type.none.fl_str_mv | Article PublishedVersion info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | ABSTRACT.The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | INIAOAI_1b3701fd61b6a7c6dd5a5655eae17a44 |
| instacron_str | Instituto Nacional de Investigación Agropecuaria |
| institution | Instituto Nacional de Investigación Agropecuaria |
| instname_str | Instituto Nacional de Investigación Agropecuaria |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | INIAOAI |
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| oai_identifier_str | oai:redi.anii.org.uy:20.500.12381/2689 |
| publishDate | 2016 |
| reponame_str | AINFO |
| repository.mail.fl_str_mv | lorrego@inia.org.uy |
| repository.name.fl_str_mv | AINFO - Instituto Nacional de Investigación Agropecuaria |
| repository_id_str | |
| rights_invalid_str_mv | Acceso abierto |
| spelling | 2022-12-16T20:51:01Z2022-12-16T20:51:01Z20162022-12-16T20:51:01Zhttp://www.ainfo.inia.uy/consulta/busca?b=pc&id=56407&biblioteca=vazio&busca=56407&qFacets=56407ABSTRACT.The anther culture response in Solanum commersonii (2n = 2x = 24, 1EBN) and S. chacoense (2n = 2x = 24, 2EBN), two wild potato germplasm resources was studied to obtain haploid plants. Three accessions from each of the two species and 3200 anthers from each genotype were cultured. Authors assessed different culture media; ascorbic acid, L?cysteine and silver nitrate (AgNO3) were included to prevent browning of anther cultures. Addition of AgNO3, was effective to induce embryogenesis. The clones from S. commersonii showed different embryogenic response to androgenesis. However, the three accessions from S. chacoense did not induce any embryo in the same conditions. Ploidy level of the regenerated clones was estimated by flow cytometry and confirmed by chromosome counts. This is the first report of haploid plants obtained from anther culture in S. commersonii, with important implications in sequencing efforts and potato breeding.https://hdl.handle.net/20.500.12381/2689enenginfo:eu-repo/semantics/openAccessAcceso abiertoHAPLOIDDOUBLE HAPLOIDANTHER CULTUREFLOW CYTOMETRYPAPASOLANUM COMMERSONIIGERMOPLASMAGametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding.ArticlePublishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:AINFOinstname:Instituto Nacional de Investigación Agropecuariainstacron:Instituto Nacional de Investigación AgropecuariaCASTILLO, A.GAIERO, P.LÓPEZ CARRO, B.VILARO, F.SWORDsword-2022-12-16T17:51:01.original.xmlOriginal SWORD entry documentapplication/octet-stream2186https://redi.anii.org.uy/jspui/bitstream/20.500.12381/2689/1/sword-2022-12-16T17%3a51%3a01.original.xmld703f843820ce52fcd137eafd4e6cac8MD5120.500.12381/26892022-12-16 17:51:02.076oai:redi.anii.org.uy:20.500.12381/2689Gobiernohttp://inia.uyhttps://redi.anii.org.uy/oai/requestlorrego@inia.org.uyUruguayopendoar:2022-12-16T20:51:02AINFO - Instituto Nacional de Investigación Agropecuariafalse |
| spellingShingle | Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. CASTILLO, A. HAPLOID DOUBLE HAPLOID ANTHER CULTURE FLOW CYTOMETRY PAPA SOLANUM COMMERSONII GERMOPLASMA |
| status_str | publishedVersion |
| title | Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
| title_full | Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
| title_fullStr | Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
| title_full_unstemmed | Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
| title_short | Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
| title_sort | Gametic embryogenic response in wild diploid Solanum species and its implications for genome sequencing projects and breeding. |
| topic | HAPLOID DOUBLE HAPLOID ANTHER CULTURE FLOW CYTOMETRY PAPA SOLANUM COMMERSONII GERMOPLASMA |
| url | http://www.ainfo.inia.uy/consulta/busca?b=pc&id=56407&biblioteca=vazio&busca=56407&qFacets=56407 |
