Most commercial apple orchards are established on either Marubakaido (Malus prunifolia Borkh.) (high vigor), M-9 (Malus pumila) (low vigor), or a combination of both rootstocks through the intergrafting technique. The Geneva® Series rootstocks were developed as an alternative to orchards modernization. The vigor of rootstocks influences the anatomical xylem formation and, therefore, the canopy cultivar's hydraulic conductivity. When affected by embolism, hydraulic conductivity harms plant metabolism, reducing crop yield and fruit quality. This study aimed to evaluate four Geneva® Series rootstocks with potential use in southern Brazil, in terms of hydraulic conductivity (K) and percentage loss of conductance (PLC), during the winter period in two different years. The G.213 rootstock presented the best performance for the variables analyzed; however, higher values of xylem functionality loss were observed in G.814.