Altering compositional properties of viral genomes to design Llve-attenuated vaccines
Resumen:
Live-attenuated vaccines have been historically used to successfully prevent numerous diseases caused by a broad variety of RNA viruses due to their ability to elicit strong and perdurable immune-protective responses. In recent years, various strategies have been explored to achieve viral attenuation by rational genetic design rather than using classic and empirical approaches, based on successive passages in cell culture. A deeper understanding of evolutionary implications of distinct viral genomic compositional aspects, as well as substantial advances in synthetic biology technologies, have provided a framework to achieve new viral attenuation strategies. Herein, we will discuss different approaches that are currently applied to modify compositional features of viruses in order to develop novel live-attenuated vaccines.
| 2021 | |
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ANII: FCE_1_2019_1_156157 ANII: FCE_1_2019_1_155930 |
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RNA viruses Genome composition Codon usage Codon pair bias Mutational robustness Attenuation, vaccines |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/40941 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución (CC - By 4.0) |
| _version_ | 1807522799901736960 |
|---|---|
| author | Pereira Gómez, Marianoel |
| author2 | Carrau Eguía, Lucía Fajardo Rossi, Álvaro Moreno Karlen, María del Pilar Moratorio, Gonzalo |
| author2_role | author author author author |
| author_facet | Pereira Gómez, Marianoel Carrau Eguía, Lucía Fajardo Rossi, Álvaro Moreno Karlen, María del Pilar Moratorio, Gonzalo |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Pereira Gómez Marianoel, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Carrau Eguía Lucía Fajardo Rossi Álvaro, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Moreno Karlen María del Pilar, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Moratorio Gonzalo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. |
| dc.creator.none.fl_str_mv | Pereira Gómez, Marianoel Carrau Eguía, Lucía Fajardo Rossi, Álvaro Moreno Karlen, María del Pilar Moratorio, Gonzalo |
| dc.date.accessioned.none.fl_str_mv | 2023-11-06T14:19:09Z |
| dc.date.available.none.fl_str_mv | 2023-11-06T14:19:09Z |
| dc.date.issued.none.fl_str_mv | 2021 |
| dc.description.abstract.none.fl_txt_mv | Live-attenuated vaccines have been historically used to successfully prevent numerous diseases caused by a broad variety of RNA viruses due to their ability to elicit strong and perdurable immune-protective responses. In recent years, various strategies have been explored to achieve viral attenuation by rational genetic design rather than using classic and empirical approaches, based on successive passages in cell culture. A deeper understanding of evolutionary implications of distinct viral genomic compositional aspects, as well as substantial advances in synthetic biology technologies, have provided a framework to achieve new viral attenuation strategies. Herein, we will discuss different approaches that are currently applied to modify compositional features of viruses in order to develop novel live-attenuated vaccines. |
| dc.description.sponsorship.none.fl_txt_mv | ANII: FCE_1_2019_1_156157 ANII: FCE_1_2019_1_155930 |
| dc.format.extent.es.fl_str_mv | 8 h. |
| dc.format.mimetype.es.fl_str_mv | application/pdf |
| dc.identifier.citation.es.fl_str_mv | Pereira Gómez, M, Carrau, L, Fajardo Rossi, Á, [y otros autores]. "Altering compositional properties of viral genomes to design live-attenuated vaccines". Frontiers in Microbiology. [en línea] 2021, 12: 676582. 8 h. DOI: 10.3389/fmicb.2021.676582. |
| dc.identifier.doi.none.fl_str_mv | 10.3389/fmicb.2021.676582 |
| dc.identifier.issn.none.fl_str_mv | 1664-302X |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/40941 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.publisher.es.fl_str_mv | Frontiers |
| dc.relation.ispartof.es.fl_str_mv | Frontiers in Microbiology, 2021, 12: 676582. |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.es.fl_str_mv | RNA viruses Genome composition Codon usage Codon pair bias Mutational robustness Attenuation, vaccines |
| dc.title.none.fl_str_mv | Altering compositional properties of viral genomes to design Llve-attenuated vaccines |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Live-attenuated vaccines have been historically used to successfully prevent numerous diseases caused by a broad variety of RNA viruses due to their ability to elicit strong and perdurable immune-protective responses. In recent years, various strategies have been explored to achieve viral attenuation by rational genetic design rather than using classic and empirical approaches, based on successive passages in cell culture. A deeper understanding of evolutionary implications of distinct viral genomic compositional aspects, as well as substantial advances in synthetic biology technologies, have provided a framework to achieve new viral attenuation strategies. Herein, we will discuss different approaches that are currently applied to modify compositional features of viruses in order to develop novel live-attenuated vaccines. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_fec95f3ae3db890390a82e41df9c6cc3 |
| identifier_str_mv | Pereira Gómez, M, Carrau, L, Fajardo Rossi, Á, [y otros autores]. "Altering compositional properties of viral genomes to design live-attenuated vaccines". Frontiers in Microbiology. [en línea] 2021, 12: 676582. 8 h. DOI: 10.3389/fmicb.2021.676582. 1664-302X 10.3389/fmicb.2021.676582 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/40941 |
| publishDate | 2021 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | mabel.seroubian@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Pereira Gómez Marianoel, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Carrau Eguía LucíaFajardo Rossi Álvaro, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Moreno Karlen María del Pilar, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Moratorio Gonzalo, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.2023-11-06T14:19:09Z2023-11-06T14:19:09Z2021Pereira Gómez, M, Carrau, L, Fajardo Rossi, Á, [y otros autores]. "Altering compositional properties of viral genomes to design live-attenuated vaccines". Frontiers in Microbiology. [en línea] 2021, 12: 676582. 8 h. DOI: 10.3389/fmicb.2021.676582.1664-302Xhttps://hdl.handle.net/20.500.12008/4094110.3389/fmicb.2021.676582Live-attenuated vaccines have been historically used to successfully prevent numerous diseases caused by a broad variety of RNA viruses due to their ability to elicit strong and perdurable immune-protective responses. In recent years, various strategies have been explored to achieve viral attenuation by rational genetic design rather than using classic and empirical approaches, based on successive passages in cell culture. A deeper understanding of evolutionary implications of distinct viral genomic compositional aspects, as well as substantial advances in synthetic biology technologies, have provided a framework to achieve new viral attenuation strategies. Herein, we will discuss different approaches that are currently applied to modify compositional features of viruses in order to develop novel live-attenuated vaccines.Submitted by Parodi Mónica (mparodi@fcien.edu.uy) on 2023-10-31T17:07:16Z No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) 103389fmicb2021676582.pdf: 535390 bytes, checksum: 2734b6ec2d43fe280f2e46ce21589819 (MD5)Approved for entry into archive by Faget Cecilia (lfaget@fcien.edu.uy) on 2023-11-06T14:06:25Z (GMT) No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) 103389fmicb2021676582.pdf: 535390 bytes, checksum: 2734b6ec2d43fe280f2e46ce21589819 (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2023-11-06T14:19:09Z (GMT). No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) 103389fmicb2021676582.pdf: 535390 bytes, checksum: 2734b6ec2d43fe280f2e46ce21589819 (MD5) Previous issue date: 2021ANII: FCE_1_2019_1_156157ANII: FCE_1_2019_1_1559308 h.application/pdfenengFrontiersFrontiers in Microbiology, 2021, 12: 676582.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. 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- Universidad de la Repúblicafalse |
| spellingShingle | Altering compositional properties of viral genomes to design Llve-attenuated vaccines Pereira Gómez, Marianoel RNA viruses Genome composition Codon usage Codon pair bias Mutational robustness Attenuation, vaccines |
| status_str | publishedVersion |
| title | Altering compositional properties of viral genomes to design Llve-attenuated vaccines |
| title_full | Altering compositional properties of viral genomes to design Llve-attenuated vaccines |
| title_fullStr | Altering compositional properties of viral genomes to design Llve-attenuated vaccines |
| title_full_unstemmed | Altering compositional properties of viral genomes to design Llve-attenuated vaccines |
| title_short | Altering compositional properties of viral genomes to design Llve-attenuated vaccines |
| title_sort | Altering compositional properties of viral genomes to design Llve-attenuated vaccines |
| topic | RNA viruses Genome composition Codon usage Codon pair bias Mutational robustness Attenuation, vaccines |
| url | https://hdl.handle.net/20.500.12008/40941 |
