In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation
Resumen:
Conjugated linoleic acid (CLA) is a prime substrate for intra-gastric nitration giving rise to the formation of nitroconjugated linoleic acid (NO2-CLA). Herein, NO2-CLA generation is demonstrated within the context of acute inflammatory responses both in vitro and in vivo. Macrophage activation resulted in dose- and time-dependent CLA nitration and also in the production of secondary electrophilic and non-electrophilic derivatives. Both exogenous NO2-CLA as well as that generated in situ, attenuated NF-κB-dependent gene expression, decreased pro-inflammatory cytokine production and up-regulated Nrf2-regulated proteins. Importantly, both CLA nitration and the corresponding downstream anti-inflammatory actions of NO2-CLA were recapitulated in a mouse peritonitis model where NO2-CLA administration decreased pro-inflammatory cytokines and inhibited leukocyte recruitment. Taken together, our results demonstrate that the formation of NO2-CLA has the potential to function as an adaptive response capable of not only modulating inflammation amplitude but also protecting neighboring tissues via the expression of Nrf2-dependent genes.
| 2018 | |
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Agencia Nacional de Investigación e Innovación (ANII) Comisión Sectorial de Investigación Científica (CSIC) |
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Nitro-fatty acid Electrophile Inflammation Macrophage Nrf2 NF-κB Nitration |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/50838 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| _version_ | 1877555157387968512 |
|---|---|
| author | Villacorta, Luis |
| author2 | Minarrieta, Lucia Salvatore, Sonia R. Khoo, Nicholas K. Rom, Oren Gao, Zhen Berman, Rebecca C. Jobbagy, Soma Li, Lihua Woodcock, Steven R. Chen, Y. Eugene Freeman, Bruce A. Ferreira, Ana M. Schopfer, Francisco J. Vitturi, Dario A. |
| author2_role | author author author author author author author author author author author author author author |
| author_facet | Villacorta, Luis Minarrieta, Lucia Salvatore, Sonia R. Khoo, Nicholas K. Rom, Oren Gao, Zhen Berman, Rebecca C. Jobbagy, Soma Li, Lihua Woodcock, Steven R. Chen, Y. Eugene Freeman, Bruce A. Ferreira, Ana M. Schopfer, Francisco J. Vitturi, Dario A. |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Villacorta Luis, University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Internal Medicine Minarrieta Lucia, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Inmunología Salvatore Sonia R., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical Biology Khoo Nicholas K., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical Biology Rom Oren, University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Internal Medicine Gao Zhen, University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Internal Medicine Berman Rebecca C., University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Internal Medicine Jobbagy Soma, University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical Biology Li Lihua, University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical Biology Woodcock Steven R., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical Biology Chen Y. Eugene, University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Cardiac Surgery Freeman Bruce A., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical Biology Ferreira Ana M., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Inmunología Schopfer Francisco J., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical Biology Vitturi Dario A., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical Biology |
| dc.creator.none.fl_str_mv | Villacorta, Luis Minarrieta, Lucia Salvatore, Sonia R. Khoo, Nicholas K. Rom, Oren Gao, Zhen Berman, Rebecca C. Jobbagy, Soma Li, Lihua Woodcock, Steven R. Chen, Y. Eugene Freeman, Bruce A. Ferreira, Ana M. Schopfer, Francisco J. Vitturi, Dario A. |
| dc.date.accessioned.none.fl_str_mv | 2025-07-31T16:14:01Z |
| dc.date.available.none.fl_str_mv | 2025-07-31T16:14:01Z |
| dc.date.issued.none.fl_str_mv | 2018 |
| dc.description.abstract.none.fl_txt_mv | Conjugated linoleic acid (CLA) is a prime substrate for intra-gastric nitration giving rise to the formation of nitroconjugated linoleic acid (NO2-CLA). Herein, NO2-CLA generation is demonstrated within the context of acute inflammatory responses both in vitro and in vivo. Macrophage activation resulted in dose- and time-dependent CLA nitration and also in the production of secondary electrophilic and non-electrophilic derivatives. Both exogenous NO2-CLA as well as that generated in situ, attenuated NF-κB-dependent gene expression, decreased pro-inflammatory cytokine production and up-regulated Nrf2-regulated proteins. Importantly, both CLA nitration and the corresponding downstream anti-inflammatory actions of NO2-CLA were recapitulated in a mouse peritonitis model where NO2-CLA administration decreased pro-inflammatory cytokines and inhibited leukocyte recruitment. Taken together, our results demonstrate that the formation of NO2-CLA has the potential to function as an adaptive response capable of not only modulating inflammation amplitude but also protecting neighboring tissues via the expression of Nrf2-dependent genes. |
| dc.description.sponsorship.none.fl_txt_mv | Agencia Nacional de Investigación e Innovación (ANII) Comisión Sectorial de Investigación Científica (CSIC) |
| dc.format.mimetype.es.fl_str_mv | application/pdf |
| dc.identifier.citation.es.fl_str_mv | VILLACORTA, L., MINARRIETA, L., SALVATORE, SR., y otros. In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation. Redox Biol [en línea] 2018, 15. DOI: 10.1016/j.redox.2018.01.005 |
| dc.identifier.doi.none.fl_str_mv | 10.1016/j.redox.2018.01.005 |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/50838 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Redox Biol. 15, 2018 |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.es.fl_str_mv | Nitro-fatty acid Electrophile Inflammation Macrophage Nrf2 NF-κB Nitration |
| dc.title.none.fl_str_mv | In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Conjugated linoleic acid (CLA) is a prime substrate for intra-gastric nitration giving rise to the formation of nitroconjugated linoleic acid (NO2-CLA). Herein, NO2-CLA generation is demonstrated within the context of acute inflammatory responses both in vitro and in vivo. Macrophage activation resulted in dose- and time-dependent CLA nitration and also in the production of secondary electrophilic and non-electrophilic derivatives. Both exogenous NO2-CLA as well as that generated in situ, attenuated NF-κB-dependent gene expression, decreased pro-inflammatory cytokine production and up-regulated Nrf2-regulated proteins. Importantly, both CLA nitration and the corresponding downstream anti-inflammatory actions of NO2-CLA were recapitulated in a mouse peritonitis model where NO2-CLA administration decreased pro-inflammatory cytokines and inhibited leukocyte recruitment. Taken together, our results demonstrate that the formation of NO2-CLA has the potential to function as an adaptive response capable of not only modulating inflammation amplitude but also protecting neighboring tissues via the expression of Nrf2-dependent genes. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_cb3651244e22acc6dc3ee0877c8f816b |
| identifier_str_mv | VILLACORTA, L., MINARRIETA, L., SALVATORE, SR., y otros. In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation. Redox Biol [en línea] 2018, 15. DOI: 10.1016/j.redox.2018.01.005 10.1016/j.redox.2018.01.005 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/50838 |
| publishDate | 2018 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | karina.camps@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Villacorta Luis, University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Internal MedicineMinarrieta Lucia, Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica InmunologíaSalvatore Sonia R., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical BiologyKhoo Nicholas K., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical BiologyRom Oren, University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Internal MedicineGao Zhen, University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Internal MedicineBerman Rebecca C., University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Internal MedicineJobbagy Soma, University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical BiologyLi Lihua, University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical BiologyWoodcock Steven R., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical BiologyChen Y. Eugene, University of Michigan Medical Center (Estados Unidos). Frankel Cardiovascular Center. Department of Cardiac SurgeryFreeman Bruce A., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical BiologyFerreira Ana M., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica InmunologíaSchopfer Francisco J., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical BiologyVitturi Dario A., University of Pittsburgh (Estados Unidos). Department of Pharmacology and Chemical Biology2025-07-31T16:14:01Z2025-07-31T16:14:01Z2018VILLACORTA, L., MINARRIETA, L., SALVATORE, SR., y otros. In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation. Redox Biol [en línea] 2018, 15. DOI: 10.1016/j.redox.2018.01.005https://hdl.handle.net/20.500.12008/5083810.1016/j.redox.2018.01.005Conjugated linoleic acid (CLA) is a prime substrate for intra-gastric nitration giving rise to the formation of nitroconjugated linoleic acid (NO2-CLA). Herein, NO2-CLA generation is demonstrated within the context of acute inflammatory responses both in vitro and in vivo. Macrophage activation resulted in dose- and time-dependent CLA nitration and also in the production of secondary electrophilic and non-electrophilic derivatives. Both exogenous NO2-CLA as well as that generated in situ, attenuated NF-κB-dependent gene expression, decreased pro-inflammatory cytokine production and up-regulated Nrf2-regulated proteins. Importantly, both CLA nitration and the corresponding downstream anti-inflammatory actions of NO2-CLA were recapitulated in a mouse peritonitis model where NO2-CLA administration decreased pro-inflammatory cytokines and inhibited leukocyte recruitment. Taken together, our results demonstrate that the formation of NO2-CLA has the potential to function as an adaptive response capable of not only modulating inflammation amplitude but also protecting neighboring tissues via the expression of Nrf2-dependent genes.Submitted by Haller Mariana (mhaller@higiene.edu.uy) on 2025-07-31T15:03:09Z No. of bitstreams: 2 license_rdf: 26539 bytes, checksum: 3b50ae24bd8bd076d49a70878a8a2d2c (MD5) In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation.pdf: 1615829 bytes, checksum: c448b074b1bcfe0444304fd6073f80e7 (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2025-07-31T16:14:01Z (GMT). No. of bitstreams: 2 license_rdf: 26539 bytes, checksum: 3b50ae24bd8bd076d49a70878a8a2d2c (MD5) In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation.pdf: 1615829 bytes, checksum: c448b074b1bcfe0444304fd6073f80e7 (MD5) Previous issue date: 2018Agencia Nacional de Investigación e Innovación (ANII)Comisión Sectorial de Investigación Científica (CSIC)application/pdfenengRedox Biol. 15, 2018Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)Nitro-fatty acidElectrophileInflammationMacrophageNrf2NF-κBNitrationIn situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammationArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaVillacorta, LuisMinarrieta, LuciaSalvatore, Sonia R.Khoo, Nicholas K.Rom, OrenGao, ZhenBerman, Rebecca C.Jobbagy, SomaLi, LihuaWoodcock, Steven R.Chen, Y. EugeneFreeman, Bruce A.Ferreira, Ana M.Schopfer, Francisco J.Vitturi, Dario A.LICENSElicense.txtlicense.txttext/plain; charset=utf-84267http://localhost:8080/xmlui/bitstream/20.500.12008/50838/5/license.txt6429389a7df7277b72b7924fdc7d47a9MD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-850http://localhost:8080/xmlui/bitstream/20.500.12008/50838/2/license_urla006180e3f5b2ad0b88185d14284c0e0MD52license_textlicense_texttext/html; charset=utf-829438http://localhost:8080/xmlui/bitstream/20.500.12008/50838/3/license_text0feedbc93c9013cb52b8473c08f41376MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-826539http://localhost:8080/xmlui/bitstream/20.500.12008/50838/4/license_rdf3b50ae24bd8bd076d49a70878a8a2d2cMD54ORIGINALIn situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation.pdfIn situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine 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13:14:01.939oai:colibri.udelar.edu.uy:20.500.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- Universidad de la Repúblicafalse |
| spellingShingle | In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation Villacorta, Luis Nitro-fatty acid Electrophile Inflammation Macrophage Nrf2 NF-κB Nitration |
| status_str | publishedVersion |
| title | In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation |
| title_full | In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation |
| title_fullStr | In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation |
| title_full_unstemmed | In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation |
| title_short | In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation |
| title_sort | In situ generation, metabolism and immunomodulatory signaling actions of nitro-conjugated linoleic acid in a murine model of inflammation |
| topic | Nitro-fatty acid Electrophile Inflammation Macrophage Nrf2 NF-κB Nitration |
| url | https://hdl.handle.net/20.500.12008/50838 |
