In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging
Resumen:
Macromolecular crowding is crucial for cellular homeostasis. In vivo studies of macromolecular crowding and water dynamics are needed to understand their roles in cellular physiology and fate determination. Macromolecular crowding in the lens is essential for normal optics, and an understanding of its regulation will help prevent cataract and presbyopia. Here, we combine the use of the nanoenvironmental sensor [6-acetyl-2-dimethylaminonaphthalene (ACDAN)] to visualize lens macromolecular crowding with in vivo studies of aquaporin 0 zebrafish mutants that disrupt its regulation. Spectral phasor analysis of ACDAN fluorescence reveals water dipolar relaxation and demonstrates that mutations in two zebrafish aquaporin 0s, Aqp0a and Aqp0b, alter water state and macromolecular crowding in living lenses. Our results provide in vivo evidence that Aqp0a promotes fluid influx in the deeper lens cortex, whereas Aqp0b facilitates fluid efflux. This evidence reveals previously unidentified spatial regulation of macromolecular crowding and spatially distinct roles for Aqp0 in the lens.
| 2022 | |
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ANIMALES GENÉTICA ACUAPORINAS PROTEÍNAS DEL OJO METABOLISMO CRISTALINO PEZ CEBRA AGUA |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/55180 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) |
| _version_ | 1877555255882809344 |
|---|---|
| author | Vorontsova, Irene |
| author2 | Vallmitjana, Alexander Torrado, Belén Schilling, Thomas F. Hall, James E. Gratton, Enrico Malacrida, Leonel |
| author2_role | author author author author author author |
| author_facet | Vorontsova, Irene Vallmitjana, Alexander Torrado, Belén Schilling, Thomas F. Hall, James E. Gratton, Enrico Malacrida, Leonel |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Vorontsova Irene, University of California San Francisco (E.E.U.U.). Developmental and Cell Biology, Physiology and Biophysics and Neurobiology and Behavior Vallmitjana Alexander, University of California San Francisco (E.E.U.U.). Biomedical Engineering Torrado Belén, University of California San Francisco (E.E.U.U.). Biomedical Engineering Schilling Thomas F., University of California San Francisco (E.E.U.U.). Developmental and Cell Biology Hall James E., University of California San Francisco (E.E.U.U.). Physiology and Biophysics Gratton Enrico, University of California San Francisco (E.E.U.U.). Biomedical Engineering Malacrida Leonel, Universidad de la República (Uruguay). Facultad de Medicina. Hospital de Clínicas. Departamento de Fisiopatología; Institut Pasteur de Montevideo (Uruguay) |
| dc.creator.none.fl_str_mv | Vorontsova, Irene Vallmitjana, Alexander Torrado, Belén Schilling, Thomas F. Hall, James E. Gratton, Enrico Malacrida, Leonel |
| dc.date.accessioned.none.fl_str_mv | 2026-05-25T18:43:53Z |
| dc.date.available.none.fl_str_mv | 2026-05-25T18:43:53Z |
| dc.date.issued.none.fl_str_mv | 2022 |
| dc.description.abstract.none.fl_txt_mv | Macromolecular crowding is crucial for cellular homeostasis. In vivo studies of macromolecular crowding and water dynamics are needed to understand their roles in cellular physiology and fate determination. Macromolecular crowding in the lens is essential for normal optics, and an understanding of its regulation will help prevent cataract and presbyopia. Here, we combine the use of the nanoenvironmental sensor [6-acetyl-2-dimethylaminonaphthalene (ACDAN)] to visualize lens macromolecular crowding with in vivo studies of aquaporin 0 zebrafish mutants that disrupt its regulation. Spectral phasor analysis of ACDAN fluorescence reveals water dipolar relaxation and demonstrates that mutations in two zebrafish aquaporin 0s, Aqp0a and Aqp0b, alter water state and macromolecular crowding in living lenses. Our results provide in vivo evidence that Aqp0a promotes fluid influx in the deeper lens cortex, whereas Aqp0b facilitates fluid efflux. This evidence reveals previously unidentified spatial regulation of macromolecular crowding and spatially distinct roles for Aqp0 in the lens. |
| dc.format.extent.es.fl_str_mv | 14 p. |
| dc.format.mimetype.es.fl_str_mv | application/pdf |
| dc.identifier.citation.es.fl_str_mv | Vorontsova I, Vallmitjana A, Torrado B y otros. In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging. Science Advances [en línea]. 2022;8(7). 14 p. |
| dc.identifier.doi.none.fl_str_mv | 10.1126/sciadv.abj4833 |
| dc.identifier.eissn.none.fl_str_mv | 2375-2548 |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/55180 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.publisher.es.fl_str_mv | American Association for the Advancement of Science |
| dc.relation.none.fl_str_mv | Science Advances. 2022;8(7) |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.other.es.fl_str_mv | ANIMALES GENÉTICA ACUAPORINAS PROTEÍNAS DEL OJO METABOLISMO CRISTALINO PEZ CEBRA AGUA |
| dc.title.none.fl_str_mv | In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Macromolecular crowding is crucial for cellular homeostasis. In vivo studies of macromolecular crowding and water dynamics are needed to understand their roles in cellular physiology and fate determination. Macromolecular crowding in the lens is essential for normal optics, and an understanding of its regulation will help prevent cataract and presbyopia. Here, we combine the use of the nanoenvironmental sensor [6-acetyl-2-dimethylaminonaphthalene (ACDAN)] to visualize lens macromolecular crowding with in vivo studies of aquaporin 0 zebrafish mutants that disrupt its regulation. Spectral phasor analysis of ACDAN fluorescence reveals water dipolar relaxation and demonstrates that mutations in two zebrafish aquaporin 0s, Aqp0a and Aqp0b, alter water state and macromolecular crowding in living lenses. Our results provide in vivo evidence that Aqp0a promotes fluid influx in the deeper lens cortex, whereas Aqp0b facilitates fluid efflux. This evidence reveals previously unidentified spatial regulation of macromolecular crowding and spatially distinct roles for Aqp0 in the lens. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_c3365a832a4fe1aa910288be4057b0fa |
| identifier_str_mv | Vorontsova I, Vallmitjana A, Torrado B y otros. In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging. Science Advances [en línea]. 2022;8(7). 14 p. 10.1126/sciadv.abj4833 2375-2548 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/55180 |
| publishDate | 2022 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | karina.camps@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0) |
| spelling | Vorontsova Irene, University of California San Francisco (E.E.U.U.). Developmental and Cell Biology, Physiology and Biophysics and Neurobiology and BehaviorVallmitjana Alexander, University of California San Francisco (E.E.U.U.). Biomedical EngineeringTorrado Belén, University of California San Francisco (E.E.U.U.). Biomedical EngineeringSchilling Thomas F., University of California San Francisco (E.E.U.U.). Developmental and Cell BiologyHall James E., University of California San Francisco (E.E.U.U.). Physiology and BiophysicsGratton Enrico, University of California San Francisco (E.E.U.U.). Biomedical EngineeringMalacrida Leonel, Universidad de la República (Uruguay). Facultad de Medicina. Hospital de Clínicas. Departamento de Fisiopatología; Institut Pasteur de Montevideo (Uruguay)2026-05-25T18:43:53Z2026-05-25T18:43:53Z2022Vorontsova I, Vallmitjana A, Torrado B y otros. In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging. Science Advances [en línea]. 2022;8(7). 14 p.https://hdl.handle.net/20.500.12008/5518010.1126/sciadv.abj48332375-2548Macromolecular crowding is crucial for cellular homeostasis. In vivo studies of macromolecular crowding and water dynamics are needed to understand their roles in cellular physiology and fate determination. Macromolecular crowding in the lens is essential for normal optics, and an understanding of its regulation will help prevent cataract and presbyopia. Here, we combine the use of the nanoenvironmental sensor [6-acetyl-2-dimethylaminonaphthalene (ACDAN)] to visualize lens macromolecular crowding with in vivo studies of aquaporin 0 zebrafish mutants that disrupt its regulation. Spectral phasor analysis of ACDAN fluorescence reveals water dipolar relaxation and demonstrates that mutations in two zebrafish aquaporin 0s, Aqp0a and Aqp0b, alter water state and macromolecular crowding in living lenses. Our results provide in vivo evidence that Aqp0a promotes fluid influx in the deeper lens cortex, whereas Aqp0b facilitates fluid efflux. This evidence reveals previously unidentified spatial regulation of macromolecular crowding and spatially distinct roles for Aqp0 in the lens.Submitted by Almiñana María Cecilia (marialminana@gmail.com) on 2026-05-25T16:27:28Z No. of bitstreams: 2 license_rdf: 26648 bytes, checksum: e9507f17d292a045f834ee111e4d098b (MD5) In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens.pdf: 27668508 bytes, checksum: 5b7daf330791b2fffd00fd9c55ef812e (MD5)Approved for entry into archive by Almiñana María Cecilia (marialminana@gmail.com) on 2026-05-25T17:57:53Z (GMT) No. of bitstreams: 2 license_rdf: 26648 bytes, checksum: e9507f17d292a045f834ee111e4d098b (MD5) In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens.pdf: 27668508 bytes, checksum: 5b7daf330791b2fffd00fd9c55ef812e (MD5)Made available in DSpace by Camps Karina (karina.camps@seciu.edu.uy) on 2026-05-25T18:43:53Z (GMT). No. of bitstreams: 2 license_rdf: 26648 bytes, checksum: e9507f17d292a045f834ee111e4d098b (MD5) In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens.pdf: 27668508 bytes, checksum: 5b7daf330791b2fffd00fd9c55ef812e (MD5) Previous issue date: 202214 p.application/pdfenengAmerican Association for the Advancement of ScienceScience Advances. 2022;8(7)Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial (CC - By-NC 4.0)ANIMALESGENÉTICAACUAPORINASPROTEÍNAS DEL OJOMETABOLISMOCRISTALINOPEZ CEBRAAGUAIn vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imagingArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaVorontsova, IreneVallmitjana, AlexanderTorrado, BelénSchilling, Thomas F.Hall, James E.Gratton, EnricoMalacrida, LeonelLICENSElicense.txtlicense.txttext/plain; charset=utf-84267http://localhost:8080/xmlui/bitstream/20.500.12008/55180/5/license.txt6429389a7df7277b72b7924fdc7d47a9MD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-847http://localhost:8080/xmlui/bitstream/20.500.12008/55180/2/license_url966d4a1cc97b2c4389b5142dd97d3c7fMD52license_textlicense_texttext/html; charset=utf-834460http://localhost:8080/xmlui/bitstream/20.500.12008/55180/3/license_texta7687849d40e295453d8da39c8aead05MD53license_rdflicense_rdfapplication/rdf+xml; charset=utf-826648http://localhost:8080/xmlui/bitstream/20.500.12008/55180/4/license_rdfe9507f17d292a045f834ee111e4d098bMD54ORIGINALIn vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens.pdfIn vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens.pdfapplication/pdf27668508http://localhost:8080/xmlui/bitstream/20.500.12008/55180/1/In+vivo+macromolecular+crowding+is+differentially+modulated+by+aquaporin+0+in+zebrafish+lens.pdf5b7daf330791b2fffd00fd9c55ef812eMD5120.500.12008/551802026-05-25 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- Universidad de la Repúblicafalse |
| spellingShingle | In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging Vorontsova, Irene ANIMALES GENÉTICA ACUAPORINAS PROTEÍNAS DEL OJO METABOLISMO CRISTALINO PEZ CEBRA AGUA |
| status_str | publishedVersion |
| title | In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging |
| title_full | In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging |
| title_fullStr | In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging |
| title_full_unstemmed | In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging |
| title_short | In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging |
| title_sort | In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging |
| topic | ANIMALES GENÉTICA ACUAPORINAS PROTEÍNAS DEL OJO METABOLISMO CRISTALINO PEZ CEBRA AGUA |
| url | https://hdl.handle.net/20.500.12008/55180 |
