Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii
Resumen:
Toxoplasma gondii is a major cause of reproductive losses in farm animals worldwide, with sheep being a particularly sensitive species. Yet, the diversity and biological characteristics of strains associated with ovine abortion remain incompletely understood in many regions, including South America whereby genetic diversity is known to be high. In this study, we investigated the genetic diversity of T. gondii associated with ovine abortion in Uruguay through genotyping and phylogenetic analyses of samples obtained from abortion cases. To place the observed diversity in a broader epidemiological context, ovine data were compared with previously reported genotypes circulating in humans locally. In addition, two novel strains were isolated from independent ovine abortion cases and subjected to determination of their infectivity both in vitro and in vivo, as well as to whole-genome sequencing. Integrated analyses revealed that the isolates represent previously undescribed genotypes and display marked differences in virulence-associated traits. Genomic analysis identified variation in loci previously linked to parasite virulence, including differences in ROP5 copy number. Together, our results provide new insight into the diversity of T. gondii associated with ovine abortion in Uruguay and expand current knowledge of the biological and genomic features of strains circulating in this species.
| 2026 | |
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Agencia Nacional de Investigación e Innovación (ANII) Fondo para la Convergencia Estructural del Mercosur (FOCEM) Instituto Nacional de Investigación Agropecuaria (INIA) Programa de Desarrollo de las Ciencias Básicas (PEDEClBA) |
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Toxoplasma gondii Sheep abortion In-silico RFLP Genotyping Genome sequencing Virulence Gene copy number variation |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/56383 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| _version_ | 1877555280013688832 |
|---|---|
| author | Tana-Hernandez, Leandro |
| author2 | Fresia, Pablo Cabrera, Andrés M. Valentin, Alejandra Dorsch, Matías Fierro, Sergio Giannitti, Federico Berná, Luisa Francia, María E. |
| author2_role | author author author author author author author author |
| author_facet | Tana-Hernandez, Leandro Fresia, Pablo Cabrera, Andrés M. Valentin, Alejandra Dorsch, Matías Fierro, Sergio Giannitti, Federico Berná, Luisa Francia, María E. |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Tana-Hernandez Leandro, Institut Pasteur de Montevideo (Uruguay). Laboratory of Apicomplexan Biology Fresia Pablo, Institut Pasteur de Montevideo (Uruguay). Unidad Mixta Pasteur + INIA (UMPI) Cabrera Andrés M., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Parasitología y Micología Valentin Alejandra, Institut Pasteur de Montevideo (Uruguay). Laboratory of Apicomplexan Biology Dorsch Matías, Instituto Nacional de Investigación Agropecuaria (INIA) (Uruguay). Estación Experimental La Estanzuela. Plataforma de Investigación en Salud Animal Fierro Sergio, Secretariado Uruguayo de la Lana (Uruguay) Giannitti Federico, Instituto Nacional de Investigación Agropecuaria (INIA) (Uruguay). Estación Experimental La Estanzuela. Plataforma de Investigación en Salud Animal Berná Luisa, Institut Pasteur de Montevideo (Uruguay). Laboratory of Apicomplexan Biology Francia María E., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Parasitología y Micología |
| dc.coverage.spatial.es.fl_str_mv | Uruguay |
| dc.creator.none.fl_str_mv | Tana-Hernandez, Leandro Fresia, Pablo Cabrera, Andrés M. Valentin, Alejandra Dorsch, Matías Fierro, Sergio Giannitti, Federico Berná, Luisa Francia, María E. |
| dc.date.accessioned.none.fl_str_mv | 2026-08-17T15:45:47Z |
| dc.date.available.none.fl_str_mv | 2026-08-17T15:45:47Z |
| dc.date.issued.none.fl_str_mv | 2026 |
| dc.description.abstract.none.fl_txt_mv | Toxoplasma gondii is a major cause of reproductive losses in farm animals worldwide, with sheep being a particularly sensitive species. Yet, the diversity and biological characteristics of strains associated with ovine abortion remain incompletely understood in many regions, including South America whereby genetic diversity is known to be high. In this study, we investigated the genetic diversity of T. gondii associated with ovine abortion in Uruguay through genotyping and phylogenetic analyses of samples obtained from abortion cases. To place the observed diversity in a broader epidemiological context, ovine data were compared with previously reported genotypes circulating in humans locally. In addition, two novel strains were isolated from independent ovine abortion cases and subjected to determination of their infectivity both in vitro and in vivo, as well as to whole-genome sequencing. Integrated analyses revealed that the isolates represent previously undescribed genotypes and display marked differences in virulence-associated traits. Genomic analysis identified variation in loci previously linked to parasite virulence, including differences in ROP5 copy number. Together, our results provide new insight into the diversity of T. gondii associated with ovine abortion in Uruguay and expand current knowledge of the biological and genomic features of strains circulating in this species. |
| dc.description.sponsorship.none.fl_txt_mv | Agencia Nacional de Investigación e Innovación (ANII) Fondo para la Convergencia Estructural del Mercosur (FOCEM) Instituto Nacional de Investigación Agropecuaria (INIA) Programa de Desarrollo de las Ciencias Básicas (PEDEClBA) |
| dc.format.mimetype.es.fl_str_mv | application/pdf |
| dc.identifier.citation.es.fl_str_mv | TANA-HERNANDEZ, L., FRESIA, P., CABRERA, AM., y otros. Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii. Sci Rep [en línea] 2026, 16. DOI: 10.1038/s41598-026-55698-0 |
| dc.identifier.doi.none.fl_str_mv | 10.1038/s41598-026-55698-0 |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/56383 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.relation.none.fl_str_mv | Sci Rep. 16, 2026 |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.es.fl_str_mv | Toxoplasma gondii Sheep abortion In-silico RFLP Genotyping Genome sequencing Virulence Gene copy number variation |
| dc.title.none.fl_str_mv | Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Toxoplasma gondii is a major cause of reproductive losses in farm animals worldwide, with sheep being a particularly sensitive species. Yet, the diversity and biological characteristics of strains associated with ovine abortion remain incompletely understood in many regions, including South America whereby genetic diversity is known to be high. In this study, we investigated the genetic diversity of T. gondii associated with ovine abortion in Uruguay through genotyping and phylogenetic analyses of samples obtained from abortion cases. To place the observed diversity in a broader epidemiological context, ovine data were compared with previously reported genotypes circulating in humans locally. In addition, two novel strains were isolated from independent ovine abortion cases and subjected to determination of their infectivity both in vitro and in vivo, as well as to whole-genome sequencing. Integrated analyses revealed that the isolates represent previously undescribed genotypes and display marked differences in virulence-associated traits. Genomic analysis identified variation in loci previously linked to parasite virulence, including differences in ROP5 copy number. Together, our results provide new insight into the diversity of T. gondii associated with ovine abortion in Uruguay and expand current knowledge of the biological and genomic features of strains circulating in this species. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_bf58f937ef989954546ff7161ffe52db |
| identifier_str_mv | TANA-HERNANDEZ, L., FRESIA, P., CABRERA, AM., y otros. Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii. Sci Rep [en línea] 2026, 16. DOI: 10.1038/s41598-026-55698-0 10.1038/s41598-026-55698-0 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/56383 |
| publishDate | 2026 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | karina.camps@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Tana-Hernandez Leandro, Institut Pasteur de Montevideo (Uruguay). Laboratory of Apicomplexan BiologyFresia Pablo, Institut Pasteur de Montevideo (Uruguay). Unidad Mixta Pasteur + INIA (UMPI)Cabrera Andrés M., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Parasitología y MicologíaValentin Alejandra, Institut Pasteur de Montevideo (Uruguay). Laboratory of Apicomplexan BiologyDorsch Matías, Instituto Nacional de Investigación Agropecuaria (INIA) (Uruguay). Estación Experimental La Estanzuela. Plataforma de Investigación en Salud AnimalFierro Sergio, Secretariado Uruguayo de la Lana (Uruguay)Giannitti Federico, Instituto Nacional de Investigación Agropecuaria (INIA) (Uruguay). Estación Experimental La Estanzuela. Plataforma de Investigación en Salud AnimalBerná Luisa, Institut Pasteur de Montevideo (Uruguay). Laboratory of Apicomplexan BiologyFrancia María E., Universidad de la República (Uruguay). Facultad de Medicina. Instituto de Higiene. Unidad Académica Parasitología y MicologíaUruguay2026-08-17T15:45:47Z2026-08-17T15:45:47Z2026TANA-HERNANDEZ, L., FRESIA, P., CABRERA, AM., y otros. Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii. Sci Rep [en línea] 2026, 16. DOI: 10.1038/s41598-026-55698-0https://hdl.handle.net/20.500.12008/5638310.1038/s41598-026-55698-0Toxoplasma gondii is a major cause of reproductive losses in farm animals worldwide, with sheep being a particularly sensitive species. Yet, the diversity and biological characteristics of strains associated with ovine abortion remain incompletely understood in many regions, including South America whereby genetic diversity is known to be high. In this study, we investigated the genetic diversity of T. gondii associated with ovine abortion in Uruguay through genotyping and phylogenetic analyses of samples obtained from abortion cases. To place the observed diversity in a broader epidemiological context, ovine data were compared with previously reported genotypes circulating in humans locally. In addition, two novel strains were isolated from independent ovine abortion cases and subjected to determination of their infectivity both in vitro and in vivo, as well as to whole-genome sequencing. Integrated analyses revealed that the isolates represent previously undescribed genotypes and display marked differences in virulence-associated traits. Genomic analysis identified variation in loci previously linked to parasite virulence, including differences in ROP5 copy number. Together, our results provide new insight into the diversity of T. gondii associated with ovine abortion in Uruguay and expand current knowledge of the biological and genomic features of strains circulating in this species.Submitted by Haller Mariana (mhaller@higiene.edu.uy) on 2026-08-17T15:39:47Z No. of bitstreams: 2 license_rdf: 27293 bytes, checksum: d62648cf14c1e37917d392ac87012955 (MD5) Characterization of ovine abortion in Uruguay.pdf: 2484772 bytes, checksum: da8ef74232a4afb4e4cbcda216793049 (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2026-08-17T15:45:47Z (GMT). No. of bitstreams: 2 license_rdf: 27293 bytes, checksum: d62648cf14c1e37917d392ac87012955 (MD5) Characterization of ovine abortion in Uruguay.pdf: 2484772 bytes, checksum: da8ef74232a4afb4e4cbcda216793049 (MD5) Previous issue date: 2026Agencia Nacional de Investigación e Innovación (ANII)Fondo para la Convergencia Estructural del Mercosur (FOCEM)Instituto Nacional de Investigación Agropecuaria (INIA)Programa de Desarrollo de las Ciencias Básicas (PEDEClBA)application/pdfenengSci Rep. 16, 2026Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)Toxoplasma gondiiSheep abortionIn-silico RFLPGenotypingGenome sequencingVirulenceGene copy number variationCharacterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondiiArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaTana-Hernandez, LeandroFresia, PabloCabrera, Andrés M.Valentin, AlejandraDorsch, MatíasFierro, SergioGiannitti, FedericoBerná, LuisaFrancia, María E.LICENSElicense.txtlicense.txttext/plain; charset=utf-84267http://localhost:8080/xmlui/bitstream/20.500.12008/56383/5/license.txt6429389a7df7277b72b7924fdc7d47a9MD55CC-LICENSElicense_urllicense_urltext/plain; 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Institucionalhttps://www.colibri.udelar.edu.uyUniversidadhttps://udelar.edu.uy/https://www.colibri.udelar.edu.uy/oai/requestkarina.camps@seciu.edu.uyUruguayopendoar:47712026-08-17T15:45:48COLIBRI - Universidad de la Repúblicafalse |
| spellingShingle | Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii Tana-Hernandez, Leandro Toxoplasma gondii Sheep abortion In-silico RFLP Genotyping Genome sequencing Virulence Gene copy number variation |
| status_str | publishedVersion |
| title | Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii |
| title_full | Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii |
| title_fullStr | Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii |
| title_full_unstemmed | Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii |
| title_short | Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii |
| title_sort | Characterization of ovine abortion in Uruguay reveals extensive non-clonal diversity and multiple evolutionary origins of Toxoplasma gondii |
| topic | Toxoplasma gondii Sheep abortion In-silico RFLP Genotyping Genome sequencing Virulence Gene copy number variation |
| url | https://hdl.handle.net/20.500.12008/56383 |
