The family of mygalomorph spiders Paratropididae Simon, 1889 is here reported for the first time for Colombia, where it is represented by three genera (Anisaspis, Paratropis, Stormtropis gen. n.) and eight species. One genus, Stormtropis, and six species constitute new taxa that are here diagnosed, described and illustrated. The geographical distribution of Paratropis papilligera FO Pickard-Cambridge, 1896 and Paratropis elicioi Dupérré, 2015 are also redescribed and expanded on the basis of new material examined. The diagnosis of the subfamily Paratropidinae, Paratropis Simon, 1889 and Anisaspis Simon, 1892 are emended including the variations of the new species. Likewise, a geographic distribution map for the entire family and a taxonomic key for the males of Paratropidinae are included. Other biogeographic, morphological, and taxonomic aspects are discussed.