A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic
Resumen:
A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts.
| 2023 | |
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COVID-19 pandemic SARS-CoV-2 Human respiratory RNA viruses Multiplex PCR-NGS Viral coinfections |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/42441 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución (CC - By 4.0) |
| _version_ | 1807522929115660288 |
|---|---|
| author | Ramos, Natalia |
| author2 | Panzera Crespo, Yanina Frabasile Giurato, Sandra Alicia Tomás Custodio, Gonzalo Martín Calleros Basilio, Lucía Marandino, Ana Techera, Claudia Grecco Patiño, Sofía Fuques Villalba, Eddie Arbiza, Juan Pérez Crossa, Ruben Gustavo Delfraro Vázquez, Adriana Beatriz Goñi Mazzitelli, Natalia Coppola, Leticia Ramas, Vivivana Morel, Noelia Chiparelli, Héctor |
| author2_role | author author author author author author author author author author author author author author author author |
| author_facet | Ramos, Natalia Panzera Crespo, Yanina Frabasile Giurato, Sandra Alicia Tomás Custodio, Gonzalo Martín Calleros Basilio, Lucía Marandino, Ana Techera, Claudia Grecco Patiño, Sofía Fuques Villalba, Eddie Arbiza, Juan Pérez Crossa, Ruben Gustavo Delfraro Vázquez, Adriana Beatriz Goñi Mazzitelli, Natalia Coppola, Leticia Ramas, Vivivana Morel, Noelia Chiparelli, Héctor |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Ramos Natalia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Panzera Crespo Yanina, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Frabasile Giurato Sandra Alicia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica. Tomás Custodio Gonzalo Martín, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Calleros Basilio Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Marandino Ana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Techera Claudia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Grecco Patiño Sofía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Fuques Villalba Eddie, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Arbiza Juan, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Pérez Crossa Ruben Gustavo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Delfraro Vázquez Adriana Beatriz, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Goñi Mazzitelli Natalia, MSP Coppola Leticia, MSP Ramas Vivivana, MSP Morel Noelia, MSP Chiparelli Héctor, MSP |
| dc.creator.none.fl_str_mv | Ramos, Natalia Panzera Crespo, Yanina Frabasile Giurato, Sandra Alicia Tomás Custodio, Gonzalo Martín Calleros Basilio, Lucía Marandino, Ana Techera, Claudia Grecco Patiño, Sofía Fuques Villalba, Eddie Arbiza, Juan Pérez Crossa, Ruben Gustavo Delfraro Vázquez, Adriana Beatriz Goñi Mazzitelli, Natalia Coppola, Leticia Ramas, Vivivana Morel, Noelia Chiparelli, Héctor |
| dc.date.accessioned.none.fl_str_mv | 2024-02-14T14:27:21Z |
| dc.date.available.none.fl_str_mv | 2024-02-14T14:27:21Z |
| dc.date.issued.none.fl_str_mv | 2023 |
| dc.description.abstract.none.fl_txt_mv | A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts. |
| dc.format.extent.es.fl_str_mv | 7 h. |
| dc.format.mimetype.es.fl_str_mv | application/pdf |
| dc.identifier.citation.es.fl_str_mv | Ramos, N, Panzera Crespo, Y, Frabasile Giurato, S [y otros autores]. "A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic". Archives of virology. [en línea] 2023 168(3): 87. 7 h. DOI: 10.1007/s00705-023-05717-6 |
| dc.identifier.doi.none.fl_str_mv | 10.1007/s00705-023-05717-6 |
| dc.identifier.issn.none.fl_str_mv | 1432-8798 |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/42441 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.publisher.es.fl_str_mv | Springer Link |
| dc.relation.ispartof.es.fl_str_mv | Archives of virology, 2023, 168(3): 87. |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.es.fl_str_mv | COVID-19 pandemic SARS-CoV-2 Human respiratory RNA viruses Multiplex PCR-NGS Viral coinfections |
| dc.title.none.fl_str_mv | A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_a7086f5357e98104ededdbdec3b2b692 |
| identifier_str_mv | Ramos, N, Panzera Crespo, Y, Frabasile Giurato, S [y otros autores]. "A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic". Archives of virology. [en línea] 2023 168(3): 87. 7 h. DOI: 10.1007/s00705-023-05717-6 1432-8798 10.1007/s00705-023-05717-6 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/42441 |
| publishDate | 2023 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | mabel.seroubian@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Ramos Natalia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Panzera Crespo Yanina, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Frabasile Giurato Sandra Alicia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica.Tomás Custodio Gonzalo Martín, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Calleros Basilio Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Marandino Ana, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Techera Claudia, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Grecco Patiño Sofía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Fuques Villalba Eddie, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Arbiza Juan, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Pérez Crossa Ruben Gustavo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Delfraro Vázquez Adriana Beatriz, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Goñi Mazzitelli Natalia, MSPCoppola Leticia, MSPRamas Vivivana, MSPMorel Noelia, MSPChiparelli Héctor, MSP2024-02-14T14:27:21Z2024-02-14T14:27:21Z2023Ramos, N, Panzera Crespo, Y, Frabasile Giurato, S [y otros autores]. "A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic". Archives of virology. [en línea] 2023 168(3): 87. 7 h. DOI: 10.1007/s00705-023-05717-61432-8798https://hdl.handle.net/20.500.12008/4244110.1007/s00705-023-05717-6A methodological approach based on reverse transcription (RT)-multiplex PCR followed by next-generation sequencing (NGS) was implemented to identify multiple respiratory RNA viruses simultaneously. A convenience sampling from respiratory surveillance and SARS-CoV-2 diagnosis in 2020 and 2021 in Montevideo, Uruguay, was analyzed. The results revealed the cocirculation of SARS-CoV-2 with human rhinovirus (hRV) A, B and C, human respiratory syncytial virus (hRSV) B, influenza A virus, and metapneumovirus B1. SARS-CoV-2 coinfections with hRV or hRSV B and influenza A virus coinfections with hRV C were identified in adults and/or children. This methodology combines the benefits of multiplex genomic amplification with the sensitivity and information provided by NGS. An advantage is that additional viral targets can be incorporated, making it a helpful tool to investigate the cocirculation and coinfections of respiratory viruses in pandemic and post-pandemic contexts.Submitted by Pintos Natalia (nataliapintosmvd@gmail.com) on 2024-02-09T17:21:39Z No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) 0.1007s00705-023-05717-6.pdf: 1112444 bytes, checksum: c52cdfd59db55bf82380d714ad99f93f (MD5)Approved for entry into archive by Faget Cecilia (lfaget@fcien.edu.uy) on 2024-02-14T14:05:39Z (GMT) No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) 0.1007s00705-023-05717-6.pdf: 1112444 bytes, checksum: c52cdfd59db55bf82380d714ad99f93f (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2024-02-14T14:27:21Z (GMT). No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) 0.1007s00705-023-05717-6.pdf: 1112444 bytes, checksum: c52cdfd59db55bf82380d714ad99f93f (MD5) Previous issue date: 20237 h.application/pdfenengSpringer LinkArchives of virology, 2023, 168(3): 87.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)COVID-19 pandemicSARS-CoV-2Human respiratory RNA virusesMultiplex PCR-NGSViral coinfectionsA multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemicArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaRamos, NataliaPanzera Crespo, YaninaFrabasile Giurato, Sandra AliciaTomás Custodio, Gonzalo MartínCalleros Basilio, LucíaMarandino, AnaTechera, ClaudiaGrecco Patiño, SofíaFuques Villalba, EddieArbiza, JuanPérez Crossa, Ruben GustavoDelfraro Vázquez, Adriana BeatrizGoñi Mazzitelli, NataliaCoppola, LeticiaRamas, VivivanaMorel, NoeliaChiparelli, HéctorLICENSElicense.txtlicense.txttext/plain; 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- Universidad de la Repúblicafalse |
| spellingShingle | A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic Ramos, Natalia COVID-19 pandemic SARS-CoV-2 Human respiratory RNA viruses Multiplex PCR-NGS Viral coinfections |
| status_str | publishedVersion |
| title | A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic |
| title_full | A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic |
| title_fullStr | A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic |
| title_full_unstemmed | A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic |
| title_short | A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic |
| title_sort | A multiplex-NGS approach to identifying respiratory RNA viruses during the COVID-19 pandemic |
| topic | COVID-19 pandemic SARS-CoV-2 Human respiratory RNA viruses Multiplex PCR-NGS Viral coinfections |
| url | https://hdl.handle.net/20.500.12008/42441 |
