Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF)
Resumen:
In the last decade Achromobacter spp. has been associated with chronic colonization in patients with cystic fibrosis (CF). Although Achromobacter xylosoxidans is the most frequent species recovered within this genus, other species such as A. ruhlandii have also been reported in these patients. Descriptions of mobile elements are scarce in Achromobacter and none of them have been originated in A. ruhlandii. The aim of this study was to report the full characterization of a plasmid which was maintained in four clonally related A. ruhlandii isolates. Between 2013 and 2015, nine A. ruhlandii isolates were recovered from a pediatric patient with CF at a hospital in Buenos Aires. Four selected clonally related isolates were sequenced by Illumina MiSeq, annotated using RAST and manually curated. The presence of a unique plasmid of 34096-bp and 50 CDS was observed in the four isolates, displaying only 1 nucleotide substitution translated into one amino acid change among them. These plasmids have a class 1 integron containing the aac-(6′)-Ib gene, a mercury resistance operon region and the relE/stbE toxin/antitoxin system. Plasmids showed 79% similarity and 99% identity with pmatvim-7 from Pseudomonas aeruginosa. This is the first full description and characterization of a plasmid from A. ruhlandii which was maintained over time.
| 2022 | |
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Achromobacter Ruhlandii Cystic fibrosis Plasmid |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/39040 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| _version_ | 1807522797402980352 |
|---|---|
| author | Steffanowski, Carla |
| author2 | Papalia, Mariana Iriarte, Andrés Langleib de Souza, Mauricio Galanternik, Laura Gutkind, Gabriel Cooper, Vaughn Ramírez, María Soledad Radice, Marcela |
| author2_role | author author author author author author author author |
| author_facet | Steffanowski, Carla Papalia, Mariana Iriarte, Andrés Langleib de Souza, Mauricio Galanternik, Laura Gutkind, Gabriel Cooper, Vaughn Ramírez, María Soledad Radice, Marcela |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Steffanowski Carla Papalia Mariana Iriarte Andrés Langleib de Souza Mauricio, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Galanternik Laura Gutkind Gabriel Cooper Vaughn Ramírez María Soledad Radice Marcela |
| dc.creator.none.fl_str_mv | Steffanowski, Carla Papalia, Mariana Iriarte, Andrés Langleib de Souza, Mauricio Galanternik, Laura Gutkind, Gabriel Cooper, Vaughn Ramírez, María Soledad Radice, Marcela |
| dc.date.accessioned.none.fl_str_mv | 2023-08-04T14:20:00Z |
| dc.date.available.none.fl_str_mv | 2023-08-04T14:20:00Z |
| dc.date.issued.none.fl_str_mv | 2022 |
| dc.description.abstract.none.fl_txt_mv | In the last decade Achromobacter spp. has been associated with chronic colonization in patients with cystic fibrosis (CF). Although Achromobacter xylosoxidans is the most frequent species recovered within this genus, other species such as A. ruhlandii have also been reported in these patients. Descriptions of mobile elements are scarce in Achromobacter and none of them have been originated in A. ruhlandii. The aim of this study was to report the full characterization of a plasmid which was maintained in four clonally related A. ruhlandii isolates. Between 2013 and 2015, nine A. ruhlandii isolates were recovered from a pediatric patient with CF at a hospital in Buenos Aires. Four selected clonally related isolates were sequenced by Illumina MiSeq, annotated using RAST and manually curated. The presence of a unique plasmid of 34096-bp and 50 CDS was observed in the four isolates, displaying only 1 nucleotide substitution translated into one amino acid change among them. These plasmids have a class 1 integron containing the aac-(6′)-Ib gene, a mercury resistance operon region and the relE/stbE toxin/antitoxin system. Plasmids showed 79% similarity and 99% identity with pmatvim-7 from Pseudomonas aeruginosa. This is the first full description and characterization of a plasmid from A. ruhlandii which was maintained over time. |
| dc.format.extent.es.fl_str_mv | 6 h. |
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| dc.identifier.citation.es.fl_str_mv | Steffanowski, C, Papalia, M, Iriarte, A, [y otros autores]. "Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF)". Revista Argentina de Microbiología. [en línea] 2022, 54(1): 3-8. 6 h. DOI: 10.1016/j.ram.2021.01.005 |
| dc.identifier.doi.none.fl_str_mv | 10.1016/j.ram.2021.01.005 |
| dc.identifier.issn.none.fl_str_mv | 0325-7541 |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/39040 |
| dc.language.iso.none.fl_str_mv | en_US eng |
| dc.publisher.es.fl_str_mv | Elsevier España |
| dc.relation.ispartof.es.fl_str_mv | Revista Argentina de Microbiología, 2022, 54(1): 3-8. |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.es.fl_str_mv | Achromobacter Ruhlandii Cystic fibrosis Plasmid |
| dc.title.none.fl_str_mv | Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF) |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | In the last decade Achromobacter spp. has been associated with chronic colonization in patients with cystic fibrosis (CF). Although Achromobacter xylosoxidans is the most frequent species recovered within this genus, other species such as A. ruhlandii have also been reported in these patients. Descriptions of mobile elements are scarce in Achromobacter and none of them have been originated in A. ruhlandii. The aim of this study was to report the full characterization of a plasmid which was maintained in four clonally related A. ruhlandii isolates. Between 2013 and 2015, nine A. ruhlandii isolates were recovered from a pediatric patient with CF at a hospital in Buenos Aires. Four selected clonally related isolates were sequenced by Illumina MiSeq, annotated using RAST and manually curated. The presence of a unique plasmid of 34096-bp and 50 CDS was observed in the four isolates, displaying only 1 nucleotide substitution translated into one amino acid change among them. These plasmids have a class 1 integron containing the aac-(6′)-Ib gene, a mercury resistance operon region and the relE/stbE toxin/antitoxin system. Plasmids showed 79% similarity and 99% identity with pmatvim-7 from Pseudomonas aeruginosa. This is the first full description and characterization of a plasmid from A. ruhlandii which was maintained over time. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_a396579a9ee07129d8074e10603d9fba |
| identifier_str_mv | Steffanowski, C, Papalia, M, Iriarte, A, [y otros autores]. "Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF)". Revista Argentina de Microbiología. [en línea] 2022, 54(1): 3-8. 6 h. DOI: 10.1016/j.ram.2021.01.005 0325-7541 10.1016/j.ram.2021.01.005 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en_US |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/39040 |
| publishDate | 2022 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | mabel.seroubian@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Steffanowski CarlaPapalia MarianaIriarte AndrésLangleib de Souza Mauricio, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Galanternik LauraGutkind GabrielCooper VaughnRamírez María SoledadRadice Marcela2023-08-04T14:20:00Z2023-08-04T14:20:00Z2022Steffanowski, C, Papalia, M, Iriarte, A, [y otros autores]. "Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF)". Revista Argentina de Microbiología. [en línea] 2022, 54(1): 3-8. 6 h. DOI: 10.1016/j.ram.2021.01.0050325-7541https://hdl.handle.net/20.500.12008/3904010.1016/j.ram.2021.01.005In the last decade Achromobacter spp. has been associated with chronic colonization in patients with cystic fibrosis (CF). Although Achromobacter xylosoxidans is the most frequent species recovered within this genus, other species such as A. ruhlandii have also been reported in these patients. Descriptions of mobile elements are scarce in Achromobacter and none of them have been originated in A. ruhlandii. The aim of this study was to report the full characterization of a plasmid which was maintained in four clonally related A. ruhlandii isolates. Between 2013 and 2015, nine A. ruhlandii isolates were recovered from a pediatric patient with CF at a hospital in Buenos Aires. Four selected clonally related isolates were sequenced by Illumina MiSeq, annotated using RAST and manually curated. The presence of a unique plasmid of 34096-bp and 50 CDS was observed in the four isolates, displaying only 1 nucleotide substitution translated into one amino acid change among them. These plasmids have a class 1 integron containing the aac-(6′)-Ib gene, a mercury resistance operon region and the relE/stbE toxin/antitoxin system. Plasmids showed 79% similarity and 99% identity with pmatvim-7 from Pseudomonas aeruginosa. This is the first full description and characterization of a plasmid from A. ruhlandii which was maintained over time.Submitted by Farías Verónica (vfarias@fcien.edu.uy) on 2023-08-04T14:11:13Z No. of bitstreams: 2 license_rdf: 23149 bytes, checksum: 1996b8461bc290aef6a27d78c67b6b52 (MD5) 101016jram202101005.pdf: 519441 bytes, checksum: 0b99e2e9c50e3a856f6539364994c9ef (MD5)Approved for entry into archive by Faget Cecilia (lfaget@fcien.edu.uy) on 2023-08-04T14:13:50Z (GMT) No. of bitstreams: 2 license_rdf: 23149 bytes, checksum: 1996b8461bc290aef6a27d78c67b6b52 (MD5) 101016jram202101005.pdf: 519441 bytes, checksum: 0b99e2e9c50e3a856f6539364994c9ef (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2023-08-04T14:20:00Z (GMT). No. of bitstreams: 2 license_rdf: 23149 bytes, checksum: 1996b8461bc290aef6a27d78c67b6b52 (MD5) 101016jram202101005.pdf: 519441 bytes, checksum: 0b99e2e9c50e3a856f6539364994c9ef (MD5) Previous issue date: 20226 h.application/pdfen_USengElsevier EspañaRevista Argentina de Microbiología, 2022, 54(1): 3-8.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)AchromobacterRuhlandiiCystic fibrosisPlasmidFull characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF)Artículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaSteffanowski, CarlaPapalia, MarianaIriarte, AndrésLangleib de Souza, MauricioGalanternik, LauraGutkind, GabrielCooper, VaughnRamírez, María SoledadRadice, MarcelaLICENSElicense.txtlicense.txttext/plain; charset=utf-84267http://localhost:8080/xmlui/bitstream/20.500.12008/39040/5/license.txt6429389a7df7277b72b7924fdc7d47a9MD55CC-LICENSElicense_urllicense_urltext/plain; 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- Universidad de la Repúblicafalse |
| spellingShingle | Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF) Steffanowski, Carla Achromobacter Ruhlandii Cystic fibrosis Plasmid |
| status_str | publishedVersion |
| title | Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF) |
| title_full | Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF) |
| title_fullStr | Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF) |
| title_full_unstemmed | Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF) |
| title_short | Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF) |
| title_sort | Full characterization of plasmids from Achromobacter ruhlandii isolates recovered from a single patient with cystic fibrosis (CF) |
| topic | Achromobacter Ruhlandii Cystic fibrosis Plasmid |
| url | https://hdl.handle.net/20.500.12008/39040 |
