Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
Resumen:
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.
| 2021 | |
| ANII: FSSA_X_2014_1_105252 | |
|
Campylobacter fetus Bovine genital campylobacteriosis qPCR Molecular diagnosis |
|
| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/33426 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| _version_ | 1807522792661319680 |
|---|---|
| author | Delpiazzo Antón, Rafael Enrique |
| author2 | Barcellos Coitiño, Maila Sabrina Barros Mayol, María Sofía Betancor, Laura Fraga Cotelo, Martín Gil, Jorge Iraola, Gregorio Morsella, C. Paolicchi, F. Pérez Crossa, Ruben Gustavo Riet-Correa, Franklin Sanguinetti, Margarita Silva Rodríguez, Alfonso da Silva Silveira, Caroline Calleros Basilio, Lucía |
| author2_role | author author author author author author author author author author author author author author |
| author_facet | Delpiazzo Antón, Rafael Enrique Barcellos Coitiño, Maila Sabrina Barros Mayol, María Sofía Betancor, Laura Fraga Cotelo, Martín Gil, Jorge Iraola, Gregorio Morsella, C. Paolicchi, F. Pérez Crossa, Ruben Gustavo Riet-Correa, Franklin Sanguinetti, Margarita Silva Rodríguez, Alfonso da Silva Silveira, Caroline Calleros Basilio, Lucía |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Delpiazzo Antón Rafael Enrique, Universidad de la República (Uruguay). Facultad de Veterinaria. Barcellos Coitiño Maila Sabrina, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Barros Mayol María Sofía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Betancor Laura, Universidad de la República (Uruguay). Facultad de Medicina. Fraga Cotelo Martín, INIA (Uruguay) Gil Jorge, Universidad de la República (Uruguay). Facultad de Veterinaria. Iraola Gregorio, Instituto Pasteur (Montevideo). Morsella C. Paolicchi Fernando Pérez Crossa Ruben Gustavo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Riet-Correa Franklin, INIA (Uruguay) Sanguinetti Margarita, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Silva Rodríguez Alfonso, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. da Silva Silveira Caroline, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Calleros Basilio Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. |
| dc.creator.none.fl_str_mv | Delpiazzo Antón, Rafael Enrique Barcellos Coitiño, Maila Sabrina Barros Mayol, María Sofía Betancor, Laura Fraga Cotelo, Martín Gil, Jorge Iraola, Gregorio Morsella, C. Paolicchi, F. Pérez Crossa, Ruben Gustavo Riet-Correa, Franklin Sanguinetti, Margarita Silva Rodríguez, Alfonso da Silva Silveira, Caroline Calleros Basilio, Lucía |
| dc.date.accessioned.none.fl_str_mv | 2022-08-30T13:55:10Z |
| dc.date.available.none.fl_str_mv | 2022-08-30T13:55:10Z |
| dc.date.issued.none.fl_str_mv | 2021 |
| dc.description.abstract.none.fl_txt_mv | Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
| dc.description.sponsorship.none.fl_txt_mv | ANII: FSSA_X_2014_1_105252 |
| dc.format.extent.es.fl_str_mv | 5 h |
| dc.format.mimetype.es.fl_str_mv | application/pdf |
| dc.identifier.citation.es.fl_str_mv | Delpiazzo Antón, R, Barcellos Coitiño, M, Barros Mayol, M [y otros autores]. "Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence". Veterinary and Animal Science. [en línea] 2021, 11: 100163. 5 h. Doi: 10.1016/j.vas.2020.100163. |
| dc.identifier.doi.none.fl_str_mv | 10.1016/j.vas.2020.100163 |
| dc.identifier.issn.none.fl_str_mv | 2451-943X |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/33426 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.publisher.es.fl_str_mv | Elsevier |
| dc.relation.ispartof.es.fl_str_mv | Veterinary and Animal Science, 2021, 11: 100163. |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.es.fl_str_mv | Campylobacter fetus Bovine genital campylobacteriosis qPCR Molecular diagnosis |
| dc.title.none.fl_str_mv | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_716b1c183cbb0e08629db5ec75d1fd9c |
| identifier_str_mv | Delpiazzo Antón, R, Barcellos Coitiño, M, Barros Mayol, M [y otros autores]. "Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence". Veterinary and Animal Science. [en línea] 2021, 11: 100163. 5 h. Doi: 10.1016/j.vas.2020.100163. 2451-943X 10.1016/j.vas.2020.100163 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/33426 |
| publishDate | 2021 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | mabel.seroubian@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0) |
| spelling | Delpiazzo Antón Rafael Enrique, Universidad de la República (Uruguay). Facultad de Veterinaria.Barcellos Coitiño Maila Sabrina, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Barros Mayol María Sofía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Betancor Laura, Universidad de la República (Uruguay). Facultad de Medicina.Fraga Cotelo Martín, INIA (Uruguay)Gil Jorge, Universidad de la República (Uruguay). Facultad de Veterinaria.Iraola Gregorio, Instituto Pasteur (Montevideo).Morsella C.Paolicchi FernandoPérez Crossa Ruben Gustavo, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Riet-Correa Franklin, INIA (Uruguay)Sanguinetti Margarita, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Silva Rodríguez Alfonso, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.da Silva Silveira Caroline, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Calleros Basilio Lucía, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.2022-08-30T13:55:10Z2022-08-30T13:55:10Z2021Delpiazzo Antón, R, Barcellos Coitiño, M, Barros Mayol, M [y otros autores]. "Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence". Veterinary and Animal Science. [en línea] 2021, 11: 100163. 5 h. Doi: 10.1016/j.vas.2020.100163.2451-943Xhttps://hdl.handle.net/20.500.12008/3342610.1016/j.vas.2020.100163Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.Submitted by Parodi Mónica (mparodi@fcien.edu.uy) on 2022-08-23T14:34:09Z No. of bitstreams: 2 license_rdf: 23149 bytes, checksum: 1996b8461bc290aef6a27d78c67b6b52 (MD5) 101016jvas2020100163.pdf: 432927 bytes, checksum: 2552a7e7e2ab4b14cf7c0966ffa5eab2 (MD5)Approved for entry into archive by Faget Cecilia (lfaget@fcien.edu.uy) on 2022-08-30T13:38:24Z (GMT) No. of bitstreams: 2 license_rdf: 23149 bytes, checksum: 1996b8461bc290aef6a27d78c67b6b52 (MD5) 101016jvas2020100163.pdf: 432927 bytes, checksum: 2552a7e7e2ab4b14cf7c0966ffa5eab2 (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2022-08-30T13:55:10Z (GMT). No. of bitstreams: 2 license_rdf: 23149 bytes, checksum: 1996b8461bc290aef6a27d78c67b6b52 (MD5) 101016jvas2020100163.pdf: 432927 bytes, checksum: 2552a7e7e2ab4b14cf7c0966ffa5eab2 (MD5) Previous issue date: 2021ANII: FSSA_X_2014_1_1052525 happlication/pdfenengElsevierVeterinary and Animal Science, 2021, 11: 100163.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución - No Comercial - Sin Derivadas (CC - By-NC-ND 4.0)Campylobacter fetusBovine genital campylobacteriosisqPCRMolecular diagnosisAccurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequenceArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaDelpiazzo Antón, Rafael EnriqueBarcellos Coitiño, Maila SabrinaBarros Mayol, María SofíaBetancor, LauraFraga Cotelo, MartínGil, JorgeIraola, GregorioMorsella, C.Paolicchi, F.Pérez Crossa, Ruben GustavoRiet-Correa, FranklinSanguinetti, MargaritaSilva Rodríguez, Alfonsoda Silva Silveira, CarolineCalleros Basilio, LucíaLICENSElicense.txtlicense.txttext/plain; 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- Universidad de la Repúblicafalse |
| spellingShingle | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence Delpiazzo Antón, Rafael Enrique Campylobacter fetus Bovine genital campylobacteriosis qPCR Molecular diagnosis |
| status_str | publishedVersion |
| title | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_full | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_fullStr | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_full_unstemmed | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_short | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| title_sort | Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence |
| topic | Campylobacter fetus Bovine genital campylobacteriosis qPCR Molecular diagnosis |
| url | https://hdl.handle.net/20.500.12008/33426 |
