Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination
Resumen:
Background: Porcine circovirus type 2 (PCV2) is a very small, non-enveloped and icosahedral virus, with circular single stranded DNA genome. This virus is the most ubiquitous and persistent pathogen currently affecting the swine industry worldwide. PCV2 has been implicated as the major causative agent of postweaning multisystemic wasting syndrome (PMWS), a disease which is characterized by severe immunosuppressive effects in the porcine host. Worldwide PCV2 isolates have been classified into four different genotypes, PCV2a, PCV2b, PCV2c and PCVd. The goal of this work was to conduct the first phylogenetic analysis of PCV2 in Chile. Methods: PCV2 partial ORF2 sequences (462 nt) obtained from 29 clinical cases of PMWS in 22 Chilean intensive swine farms, covering over the 90% of the local pork-production, were analyzed. Results: 14% and 52% of sequences belonged to the genotypes PCV2a and PCV2b, respectively. Surprisingly, 34% of sequences were PCV2a/PCV2d recombinant viruses. Conclusions: Our findings suggested that a novel cluster of Chilean sequences emerged resulting from intergenotypic recombination between PCV2a and PCV2d.
| 2017 | |
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Genetic diversity PCV2 PMWS Recombination |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/22080 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución (CC –BY 4.0) |
| _version_ | 1807522781204578304 |
|---|---|
| author | Neira, V. |
| author2 | Ramos, Natalia Tapia, R. Arbiza, Juan Neira-Carrillo, A. Quezada, M. Ruiz, Á. Bucarey, S. A. |
| author2_role | author author author author author author author |
| author_facet | Neira, V. Ramos, Natalia Tapia, R. Arbiza, Juan Neira-Carrillo, A. Quezada, M. Ruiz, Á. Bucarey, S. A. |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.es.fl_str_mv | Ramos, Natalia. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química Biológica Arbiza, Juan. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología |
| dc.creator.none.fl_str_mv | Neira, V. Ramos, Natalia Tapia, R. Arbiza, Juan Neira-Carrillo, A. Quezada, M. Ruiz, Á. Bucarey, S. A. |
| dc.date.accessioned.none.fl_str_mv | 2019-10-02T22:14:46Z |
| dc.date.available.none.fl_str_mv | 2019-10-02T22:14:46Z |
| dc.date.issued.es.fl_str_mv | 2017 |
| dc.date.submitted.es.fl_str_mv | 20191001 |
| dc.description.abstract.none.fl_txt_mv | Background: Porcine circovirus type 2 (PCV2) is a very small, non-enveloped and icosahedral virus, with circular single stranded DNA genome. This virus is the most ubiquitous and persistent pathogen currently affecting the swine industry worldwide. PCV2 has been implicated as the major causative agent of postweaning multisystemic wasting syndrome (PMWS), a disease which is characterized by severe immunosuppressive effects in the porcine host. Worldwide PCV2 isolates have been classified into four different genotypes, PCV2a, PCV2b, PCV2c and PCVd. The goal of this work was to conduct the first phylogenetic analysis of PCV2 in Chile. Methods: PCV2 partial ORF2 sequences (462 nt) obtained from 29 clinical cases of PMWS in 22 Chilean intensive swine farms, covering over the 90% of the local pork-production, were analyzed. Results: 14% and 52% of sequences belonged to the genotypes PCV2a and PCV2b, respectively. Surprisingly, 34% of sequences were PCV2a/PCV2d recombinant viruses. Conclusions: Our findings suggested that a novel cluster of Chilean sequences emerged resulting from intergenotypic recombination between PCV2a and PCV2d. |
| dc.format.mimetype.es.fl_str_mv | application/pdf |
| dc.identifier.citation.es.fl_str_mv | Neira, V., et al. Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination. Virology Journal, 2017, 14 (1), art. no. 191. doi: 10.1186/s12985-017-0850-1 |
| dc.identifier.doi.es.fl_str_mv | 10.1186/s12985-017-0850-1 |
| dc.identifier.issn.es.fl_str_mv | 1743-422X |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/22080 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.publisher.es.fl_str_mv | BioMed Central Ltd. |
| dc.relation.ispartof.es.fl_str_mv | Virology Journal, 2017, 14 (1), art. no. 191 |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución (CC –BY 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.es.fl_str_mv | Genetic diversity PCV2 PMWS Recombination |
| dc.title.none.fl_str_mv | Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Background: Porcine circovirus type 2 (PCV2) is a very small, non-enveloped and icosahedral virus, with circular single stranded DNA genome. This virus is the most ubiquitous and persistent pathogen currently affecting the swine industry worldwide. PCV2 has been implicated as the major causative agent of postweaning multisystemic wasting syndrome (PMWS), a disease which is characterized by severe immunosuppressive effects in the porcine host. Worldwide PCV2 isolates have been classified into four different genotypes, PCV2a, PCV2b, PCV2c and PCVd. The goal of this work was to conduct the first phylogenetic analysis of PCV2 in Chile. Methods: PCV2 partial ORF2 sequences (462 nt) obtained from 29 clinical cases of PMWS in 22 Chilean intensive swine farms, covering over the 90% of the local pork-production, were analyzed. Results: 14% and 52% of sequences belonged to the genotypes PCV2a and PCV2b, respectively. Surprisingly, 34% of sequences were PCV2a/PCV2d recombinant viruses. Conclusions: Our findings suggested that a novel cluster of Chilean sequences emerged resulting from intergenotypic recombination between PCV2a and PCV2d. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_515d52ad082044925189aedf7bbf3cdf |
| identifier_str_mv | Neira, V., et al. Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination. Virology Journal, 2017, 14 (1), art. no. 191. doi: 10.1186/s12985-017-0850-1 1743-422X 10.1186/s12985-017-0850-1 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/22080 |
| publishDate | 2017 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | mabel.seroubian@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC –BY 4.0) |
| spelling | Ramos, Natalia. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Química BiológicaArbiza, Juan. Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología2019-10-02T22:14:46Z2019-10-02T22:14:46Z201720191001Neira, V., et al. Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination. Virology Journal, 2017, 14 (1), art. no. 191. doi: 10.1186/s12985-017-0850-11743-422Xhttps://hdl.handle.net/20.500.12008/2208010.1186/s12985-017-0850-1Background: Porcine circovirus type 2 (PCV2) is a very small, non-enveloped and icosahedral virus, with circular single stranded DNA genome. This virus is the most ubiquitous and persistent pathogen currently affecting the swine industry worldwide. PCV2 has been implicated as the major causative agent of postweaning multisystemic wasting syndrome (PMWS), a disease which is characterized by severe immunosuppressive effects in the porcine host. Worldwide PCV2 isolates have been classified into four different genotypes, PCV2a, PCV2b, PCV2c and PCVd. The goal of this work was to conduct the first phylogenetic analysis of PCV2 in Chile. Methods: PCV2 partial ORF2 sequences (462 nt) obtained from 29 clinical cases of PMWS in 22 Chilean intensive swine farms, covering over the 90% of the local pork-production, were analyzed. Results: 14% and 52% of sequences belonged to the genotypes PCV2a and PCV2b, respectively. Surprisingly, 34% of sequences were PCV2a/PCV2d recombinant viruses. Conclusions: Our findings suggested that a novel cluster of Chilean sequences emerged resulting from intergenotypic recombination between PCV2a and PCV2d.Made available in DSpace on 2019-10-02T22:14:46Z (GMT). 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Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC –BY 4.0)Genetic diversityPCV2PMWSRecombinationGenetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombinationArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaNeira, V.Ramos, NataliaTapia, R.Arbiza, JuanNeira-Carrillo, A.Quezada, M.Ruiz, Á.Bucarey, S. 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- Universidad de la Repúblicafalse |
| spellingShingle | Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination Neira, V. Genetic diversity PCV2 PMWS Recombination |
| status_str | publishedVersion |
| title | Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination |
| title_full | Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination |
| title_fullStr | Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination |
| title_full_unstemmed | Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination |
| title_short | Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination |
| title_sort | Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination |
| topic | Genetic diversity PCV2 PMWS Recombination |
| url | https://hdl.handle.net/20.500.12008/22080 |
