Development of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levels
Resumen:
Oxygen (O2) contributes in part to the cytotoxic response in macrophages. Over the past decade, several probes have been developed for the selective detection of different oxidants in biological systems. Peroxynitrite (ONOO–), the product of the reaction between nitric oxide (●NO) and superoxide (O2●–), is a strong oxidant that can cause biological damage through oxidation and nitration. Typically used boron-based probes for peroxynitrite detection are prone to oxygen-dependent quenching by pO₂ variations and are highly pH sensitive. Therefore, we are developing a group of fluorescent boronate-based probes (Red-BI and its structural analogues Red-BII and Red-BIII, bearing an extended π-conjugated system) derived from xanthene (Scheme 1). These probes will complement and expand the use of conventional boronates, enabling accurate cellular detection of peroxynitrite and offering new insights into the role of oxygen in oxidant production within the cellular microenvironment.
| 2025 | |
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COLORANTES FLUORESCENTES ÁCIDO PEROXINITROSO NIVEL DE OXÍGENO MACRÓFAGOS SUPERÓXIDOS OXIDANTES CITOTOXINAS |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/52928 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución (CC - By 4.0) |
| _version_ | 1875692817664180224 |
|---|---|
| author | Pereyra Domenech, Josefina |
| author2 | Prolo, Carolina Radi, Rafael Ríos, Natalia |
| author2_role | author author author |
| author_facet | Pereyra Domenech, Josefina Prolo, Carolina Radi, Rafael Ríos, Natalia |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Pereyra Domenech Josefina, Universidad de la República (Uruguay). Facultad de Medicina Prolo Carolina, Universidad de la República (Uruguay). Facultad de Medicina Radi Rafael, Universidad de la República (Uruguay). Facultad de Medicina Ríos Natalia, Universidad de la República (Uruguay). Facultad de Medicina |
| dc.creator.none.fl_str_mv | Pereyra Domenech, Josefina Prolo, Carolina Radi, Rafael Ríos, Natalia |
| dc.date.accessioned.none.fl_str_mv | 2025-12-10T14:31:30Z |
| dc.date.available.none.fl_str_mv | 2025-12-10T14:31:30Z |
| dc.date.issued.none.fl_str_mv | 2025 |
| dc.description.abstract.none.fl_txt_mv | Oxygen (O2) contributes in part to the cytotoxic response in macrophages. Over the past decade, several probes have been developed for the selective detection of different oxidants in biological systems. Peroxynitrite (ONOO–), the product of the reaction between nitric oxide (●NO) and superoxide (O2●–), is a strong oxidant that can cause biological damage through oxidation and nitration. Typically used boron-based probes for peroxynitrite detection are prone to oxygen-dependent quenching by pO₂ variations and are highly pH sensitive. Therefore, we are developing a group of fluorescent boronate-based probes (Red-BI and its structural analogues Red-BII and Red-BIII, bearing an extended π-conjugated system) derived from xanthene (Scheme 1). These probes will complement and expand the use of conventional boronates, enabling accurate cellular detection of peroxynitrite and offering new insights into the role of oxygen in oxidant production within the cellular microenvironment. |
| dc.description.es.fl_txt_mv | Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.-- Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.-- Programa en Alimentos y Salud Humana (PAyS), Facultad de Medicina, Universidad de la República. |
| dc.format.extent.es.fl_str_mv | 1 p. |
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| dc.identifier.citation.es.fl_str_mv | Pereyra J, Prolo C, Radi R y otros. Development of Red-B, a Novel Peroxynitrite Probe for Unbiased Analysis of Oxygen-Dependent Oxidative Responses. En: 32nd Annual Conference of the Society for Redox Biology & Medicine, SfRBM meeting 2025. 19-22 de noviembre 2025; Washington DC., Estados Unidos. Montevideo: Udelar.FM, 2025. 1 p. |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/52928 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.publisher.es.fl_str_mv | Udelar. FM |
| dc.relation.none.fl_str_mv | 32nd Annual Conference of the Society for Redox Biology & Medicine. 19 al 22 de noviembre 2025, Washington DC., Estados Unidos |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.other.es.fl_str_mv | COLORANTES FLUORESCENTES ÁCIDO PEROXINITROSO NIVEL DE OXÍGENO MACRÓFAGOS SUPERÓXIDOS OXIDANTES CITOTOXINAS |
| dc.title.none.fl_str_mv | Development of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levels |
| dc.type.es.fl_str_mv | Imagen fija |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/other |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.-- Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.-- Programa en Alimentos y Salud Humana (PAyS), Facultad de Medicina, Universidad de la República. |
| eu_rights_str_mv | openAccess |
| format | other |
| id | COLIBRI_4f15aafaa3c42b237d28c7e5c6ab4237 |
| identifier_str_mv | Pereyra J, Prolo C, Radi R y otros. Development of Red-B, a Novel Peroxynitrite Probe for Unbiased Analysis of Oxygen-Dependent Oxidative Responses. En: 32nd Annual Conference of the Society for Redox Biology & Medicine, SfRBM meeting 2025. 19-22 de noviembre 2025; Washington DC., Estados Unidos. Montevideo: Udelar.FM, 2025. 1 p. |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/52928 |
| publishDate | 2025 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | karina.camps@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Pereyra Domenech Josefina, Universidad de la República (Uruguay). Facultad de MedicinaProlo Carolina, Universidad de la República (Uruguay). Facultad de MedicinaRadi Rafael, Universidad de la República (Uruguay). Facultad de MedicinaRíos Natalia, Universidad de la República (Uruguay). Facultad de Medicina2025-12-10T14:31:30Z2025-12-10T14:31:30Z2025Pereyra J, Prolo C, Radi R y otros. Development of Red-B, a Novel Peroxynitrite Probe for Unbiased Analysis of Oxygen-Dependent Oxidative Responses. En: 32nd Annual Conference of the Society for Redox Biology & Medicine, SfRBM meeting 2025. 19-22 de noviembre 2025; Washington DC., Estados Unidos. Montevideo: Udelar.FM, 2025. 1 p.https://hdl.handle.net/20.500.12008/52928Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.-- Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina, Universidad de la República, Montevideo, Uruguay.-- Programa en Alimentos y Salud Humana (PAyS), Facultad de Medicina, Universidad de la República.Oxygen (O2) contributes in part to the cytotoxic response in macrophages. Over the past decade, several probes have been developed for the selective detection of different oxidants in biological systems. Peroxynitrite (ONOO–), the product of the reaction between nitric oxide (●NO) and superoxide (O2●–), is a strong oxidant that can cause biological damage through oxidation and nitration. Typically used boron-based probes for peroxynitrite detection are prone to oxygen-dependent quenching by pO₂ variations and are highly pH sensitive. Therefore, we are developing a group of fluorescent boronate-based probes (Red-BI and its structural analogues Red-BII and Red-BIII, bearing an extended π-conjugated system) derived from xanthene (Scheme 1). These probes will complement and expand the use of conventional boronates, enabling accurate cellular detection of peroxynitrite and offering new insights into the role of oxygen in oxidant production within the cellular microenvironment.Submitted by Almiñana María Cecilia (marialminana@gmail.com) on 2025-12-09T17:28:26Z No. of bitstreams: 2 license_rdf: 25630 bytes, checksum: e7132498e7c1fe99f7096667baa99b25 (MD5) poster SfRBM meeting 2025.pdf: 4996580 bytes, checksum: 2b50baf12cf1c2ab42eccec70dbb15e6 (MD5)Approved for entry into archive by Almiñana María Cecilia (marialminana@gmail.com) on 2025-12-10T14:22:24Z (GMT) No. of bitstreams: 2 license_rdf: 25630 bytes, checksum: e7132498e7c1fe99f7096667baa99b25 (MD5) poster SfRBM meeting 2025.pdf: 4996580 bytes, checksum: 2b50baf12cf1c2ab42eccec70dbb15e6 (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2025-12-10T14:31:30Z (GMT). No. of bitstreams: 2 license_rdf: 25630 bytes, checksum: e7132498e7c1fe99f7096667baa99b25 (MD5) poster SfRBM meeting 2025.pdf: 4996580 bytes, checksum: 2b50baf12cf1c2ab42eccec70dbb15e6 (MD5) Previous issue date: 20251 p.application/pdfenengUdelar. FM32nd Annual Conference of the Society for Redox Biology & Medicine. 19 al 22 de noviembre 2025, Washington DC., Estados UnidosLas obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)COLORANTES FLUORESCENTESÁCIDO PEROXINITROSONIVEL DE OXÍGENOMACRÓFAGOSSUPERÓXIDOSOXIDANTESCITOTOXINASDevelopment of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levelsImagen fijainfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaPereyra Domenech, JosefinaProlo, CarolinaRadi, RafaelRíos, NataliaLICENSElicense.txtlicense.txttext/plain; charset=utf-84267http://localhost:8080/xmlui/bitstream/20.500.12008/52928/5/license.txt6429389a7df7277b72b7924fdc7d47a9MD55CC-LICENSElicense_urllicense_urltext/plain; charset=utf-844http://localhost:8080/xmlui/bitstream/20.500.12008/52928/2/license_urla0ebbeafb9d2ec7cbb19d7137ebc392cMD52license_textlicense_texttext/html; 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- Universidad de la Repúblicafalse |
| spellingShingle | Development of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levels Pereyra Domenech, Josefina COLORANTES FLUORESCENTES ÁCIDO PEROXINITROSO NIVEL DE OXÍGENO MACRÓFAGOS SUPERÓXIDOS OXIDANTES CITOTOXINAS |
| status_str | publishedVersion |
| title | Development of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levels |
| title_full | Development of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levels |
| title_fullStr | Development of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levels |
| title_full_unstemmed | Development of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levels |
| title_short | Development of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levels |
| title_sort | Development of Red-B, a Peroxynitrite Probe for robust fluorimetric analysis under varying physiological pH and oxygen levels |
| topic | COLORANTES FLUORESCENTES ÁCIDO PEROXINITROSO NIVEL DE OXÍGENO MACRÓFAGOS SUPERÓXIDOS OXIDANTES CITOTOXINAS |
| url | https://hdl.handle.net/20.500.12008/52928 |
