Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil
Resumen:
Mealybugs are insects belonging to the family Pseudococcidae. This family includes many plant-pest species with similar morphologies, which may lead to errors in mealybug identification and delimitation. In the present study, we employed molecular-species-delimitation approaches based on distance (ASAP) and coalescence (GMYC and mPTP) methods to identify mealybugs collected from coffee and other plant hosts in the states of Espírito Santo, Bahia, Minas Gerais, and Pernambuco, Brazil. We obtained 171 new COI sequences, and 565 from the BOLD Systems database, representing 26 candidate species of Pseudococcidae. The MOTUs estimated were not congruent across different methods (ASAP-25; GMYC-30; mPTP-22). Misidentifications were revealed in the sequences from the BOLD Systems database involving Phenacoccus solani × Ph. solenopsis, Ph. tucumanus × Ph. baccharidis, and Planacoccus citri × Pl. minor species. Ten mealybug species were collected from coffee plants in Espírito Santo. Due to the incorrect labeling of the species sequences, the COI barcode library of the dataset from the database needs to be carefully analyzed to avoid the misidentification of species. The systematics and taxonomy of mealybugs may be improved by integrative taxonomy which may facilitate the integrated pest management of these pests.
| 2023 | |
|
Biodiversity Agricultural pests DNA barcoding Species identification Coffee plant Phylogenetic analysis Entomology Systematics Integrative taxonomy |
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| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/43268 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución (CC - By 4.0) |
| _version_ | 1807522809047416832 |
|---|---|
| author | Viana Oliveira, Pablo |
| author2 | Rosa dos Santos, Alexandre Lopes Olive, Emily Bianchi Britto, Karolinni Alves Nogueira de Almeida, Francine Pacheco da Silva, Vitor Cezar Barros Machado, Carolina Fornazier, Maurício José Ventura, José Aires Culik, Mark Paul Gaburro Paneto, Greiciane |
| author2_role | author author author author author author author author author author |
| author_facet | Viana Oliveira, Pablo Rosa dos Santos, Alexandre Lopes Olive, Emily Bianchi Britto, Karolinni Alves Nogueira de Almeida, Francine Pacheco da Silva, Vitor Cezar Barros Machado, Carolina Fornazier, Maurício José Ventura, José Aires Culik, Mark Paul Gaburro Paneto, Greiciane |
| author_role | author |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Viana Oliveira Pablo Rosa dos Santos Alexandre Lopes Olive Emily Bianchi Britto Karolinni Alves Nogueira de Almeida Francine Pacheco da Silva Vitor Cezar, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología. Barros Machado Carolina Fornazier Maurício José Ventura José Aires Culik Mark Paul Gaburro Paneto Greiciane |
| dc.creator.none.fl_str_mv | Viana Oliveira, Pablo Rosa dos Santos, Alexandre Lopes Olive, Emily Bianchi Britto, Karolinni Alves Nogueira de Almeida, Francine Pacheco da Silva, Vitor Cezar Barros Machado, Carolina Fornazier, Maurício José Ventura, José Aires Culik, Mark Paul Gaburro Paneto, Greiciane |
| dc.date.accessioned.none.fl_str_mv | 2024-04-01T13:30:51Z |
| dc.date.available.none.fl_str_mv | 2024-04-01T13:30:51Z |
| dc.date.issued.none.fl_str_mv | 2023 |
| dc.description.abstract.none.fl_txt_mv | Mealybugs are insects belonging to the family Pseudococcidae. This family includes many plant-pest species with similar morphologies, which may lead to errors in mealybug identification and delimitation. In the present study, we employed molecular-species-delimitation approaches based on distance (ASAP) and coalescence (GMYC and mPTP) methods to identify mealybugs collected from coffee and other plant hosts in the states of Espírito Santo, Bahia, Minas Gerais, and Pernambuco, Brazil. We obtained 171 new COI sequences, and 565 from the BOLD Systems database, representing 26 candidate species of Pseudococcidae. The MOTUs estimated were not congruent across different methods (ASAP-25; GMYC-30; mPTP-22). Misidentifications were revealed in the sequences from the BOLD Systems database involving Phenacoccus solani × Ph. solenopsis, Ph. tucumanus × Ph. baccharidis, and Planacoccus citri × Pl. minor species. Ten mealybug species were collected from coffee plants in Espírito Santo. Due to the incorrect labeling of the species sequences, the COI barcode library of the dataset from the database needs to be carefully analyzed to avoid the misidentification of species. The systematics and taxonomy of mealybugs may be improved by integrative taxonomy which may facilitate the integrated pest management of these pests. |
| dc.format.extent.es.fl_str_mv | 11 h. |
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| dc.identifier.citation.es.fl_str_mv | Viana Oliveira, P, Rosa dos Santos, A, Lopes Olive, E y otros. "Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil". Diversity. [en línea] 2023, 15(2): 305. 11 h. DOI: 10.3390/d15020305. |
| dc.identifier.doi.none.fl_str_mv | 10.3390/d15020305 |
| dc.identifier.issn.none.fl_str_mv | 1424-2818 |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/43268 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.publisher.es.fl_str_mv | MDPI |
| dc.relation.ispartof.es.fl_str_mv | Diversity, 2023, 15(2): 305 |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.es.fl_str_mv | Biodiversity Agricultural pests DNA barcoding Species identification Coffee plant Phylogenetic analysis Entomology Systematics Integrative taxonomy |
| dc.title.none.fl_str_mv | Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Mealybugs are insects belonging to the family Pseudococcidae. This family includes many plant-pest species with similar morphologies, which may lead to errors in mealybug identification and delimitation. In the present study, we employed molecular-species-delimitation approaches based on distance (ASAP) and coalescence (GMYC and mPTP) methods to identify mealybugs collected from coffee and other plant hosts in the states of Espírito Santo, Bahia, Minas Gerais, and Pernambuco, Brazil. We obtained 171 new COI sequences, and 565 from the BOLD Systems database, representing 26 candidate species of Pseudococcidae. The MOTUs estimated were not congruent across different methods (ASAP-25; GMYC-30; mPTP-22). Misidentifications were revealed in the sequences from the BOLD Systems database involving Phenacoccus solani × Ph. solenopsis, Ph. tucumanus × Ph. baccharidis, and Planacoccus citri × Pl. minor species. Ten mealybug species were collected from coffee plants in Espírito Santo. Due to the incorrect labeling of the species sequences, the COI barcode library of the dataset from the database needs to be carefully analyzed to avoid the misidentification of species. The systematics and taxonomy of mealybugs may be improved by integrative taxonomy which may facilitate the integrated pest management of these pests. |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_3943e1c99aa53df9c0c3b96416ece1ef |
| identifier_str_mv | Viana Oliveira, P, Rosa dos Santos, A, Lopes Olive, E y otros. "Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil". Diversity. [en línea] 2023, 15(2): 305. 11 h. DOI: 10.3390/d15020305. 1424-2818 10.3390/d15020305 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/43268 |
| publishDate | 2023 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | mabel.seroubian@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Viana Oliveira PabloRosa dos Santos AlexandreLopes Olive EmilyBianchi Britto KarolinniAlves Nogueira de Almeida FrancinePacheco da Silva Vitor Cezar, Universidad de la República (Uruguay). Facultad de Ciencias. Instituto de Biología.Barros Machado CarolinaFornazier Maurício JoséVentura José AiresCulik Mark PaulGaburro Paneto Greiciane2024-04-01T13:30:51Z2024-04-01T13:30:51Z2023Viana Oliveira, P, Rosa dos Santos, A, Lopes Olive, E y otros. "Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil". Diversity. [en línea] 2023, 15(2): 305. 11 h. DOI: 10.3390/d15020305.1424-2818https://hdl.handle.net/20.500.12008/4326810.3390/d15020305Mealybugs are insects belonging to the family Pseudococcidae. This family includes many plant-pest species with similar morphologies, which may lead to errors in mealybug identification and delimitation. In the present study, we employed molecular-species-delimitation approaches based on distance (ASAP) and coalescence (GMYC and mPTP) methods to identify mealybugs collected from coffee and other plant hosts in the states of Espírito Santo, Bahia, Minas Gerais, and Pernambuco, Brazil. We obtained 171 new COI sequences, and 565 from the BOLD Systems database, representing 26 candidate species of Pseudococcidae. The MOTUs estimated were not congruent across different methods (ASAP-25; GMYC-30; mPTP-22). Misidentifications were revealed in the sequences from the BOLD Systems database involving Phenacoccus solani × Ph. solenopsis, Ph. tucumanus × Ph. baccharidis, and Planacoccus citri × Pl. minor species. Ten mealybug species were collected from coffee plants in Espírito Santo. Due to the incorrect labeling of the species sequences, the COI barcode library of the dataset from the database needs to be carefully analyzed to avoid the misidentification of species. The systematics and taxonomy of mealybugs may be improved by integrative taxonomy which may facilitate the integrated pest management of these pests.Submitted by Pintos Natalia (nataliapintosmvd@gmail.com) on 2024-03-22T15:38:26Z No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) 10.3390.d15020305.pdf: 2528040 bytes, checksum: 4cc5f855536ef954364710555e062339 (MD5)Approved for entry into archive by Faget Cecilia (lfaget@fcien.edu.uy) on 2024-04-01T12:35:58Z (GMT) No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) 10.3390.d15020305.pdf: 2528040 bytes, checksum: 4cc5f855536ef954364710555e062339 (MD5)Made available in DSpace by Seroubian Mabel (mabel.seroubian@seciu.edu.uy) on 2024-04-01T13:30:51Z (GMT). No. of bitstreams: 2 license_rdf: 24251 bytes, checksum: 71ed42ef0a0b648670f707320be37b90 (MD5) 10.3390.d15020305.pdf: 2528040 bytes, checksum: 4cc5f855536ef954364710555e062339 (MD5) Previous issue date: 202311 h.application/pdfenengMDPIDiversity, 2023, 15(2): 305Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. Nº 16 de C.D.C. de 07/10/2014)info:eu-repo/semantics/openAccessLicencia Creative Commons Atribución (CC - By 4.0)BiodiversityAgricultural pestsDNA barcodingSpecies identificationCoffee plantPhylogenetic analysisEntomologySystematicsIntegrative taxonomyMolecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, BrazilArtículoinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionreponame:COLIBRIinstname:Universidad de la Repúblicainstacron:Universidad de la RepúblicaViana Oliveira, PabloRosa dos Santos, AlexandreLopes Olive, EmilyBianchi Britto, KarolinniAlves Nogueira de Almeida, FrancinePacheco da Silva, Vitor CezarBarros Machado, CarolinaFornazier, Maurício JoséVentura, José AiresCulik, Mark PaulGaburro Paneto, GreicianeLICENSElicense.txtlicense.txttext/plain; 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- Universidad de la Repúblicafalse |
| spellingShingle | Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil Viana Oliveira, Pablo Biodiversity Agricultural pests DNA barcoding Species identification Coffee plant Phylogenetic analysis Entomology Systematics Integrative taxonomy |
| status_str | publishedVersion |
| title | Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil |
| title_full | Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil |
| title_fullStr | Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil |
| title_full_unstemmed | Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil |
| title_short | Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil |
| title_sort | Molecular species delimitation using COI barcodes of mealybugs (Hemiptera: Pseudococcidae) from coffee plants in Espírito Santo, Brazil |
| topic | Biodiversity Agricultural pests DNA barcoding Species identification Coffee plant Phylogenetic analysis Entomology Systematics Integrative taxonomy |
| url | https://hdl.handle.net/20.500.12008/43268 |
