Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing
Resumen:
This scientific study focuses on tuberculosis (TB) within prison settings, where persons deprived of liberty (PDL) face significantly higher rates of the disease compared to the general population. The research employs the low-cost amplicon sequencing of Mycobacterium tuberculosis strains, aiming first to identify specific lineages and also to detect mutations associated with drug resistance. The method involves multiplex amplification, DNA extraction, and sequencing, providing valuable insights into TB dynamics and resistance-mutation profiles within the prison system at an affordable cost. The study identifies a characteristic lineage (X) circulating among PDLs in the penitentiary system in Uruguay, absent in the general population, and notes its prevalence at prison entry. No high-confidence mutations associated with drug resistance were found. The findings underscore the importance of molecular epidemiology in TB control, emphasizing the potential for intra-prison transmissions and the need for broader studies to understand strain dynamics.
| 2024 | |
| ANII: FMV_3_2018_1_148367 | |
|
Tuberculosis Epidemiology Amplicon sequencing |
|
| Inglés | |
| Universidad de la República | |
| COLIBRI | |
| https://hdl.handle.net/20.500.12008/51034 | |
| Acceso abierto | |
| Licencia Creative Commons Atribución (CC - By 4.0) |
| _version_ | 1872864700750888960 |
|---|---|
| author | Hurtado Gutiérrez, Joaquín Ignacio |
| author2 | Bentancor, María Noel Laserra, Paula Coitinho, Cecilia Greif, Gonzalo |
| author2_role | author author author author |
| author_facet | Hurtado Gutiérrez, Joaquín Ignacio Bentancor, María Noel Laserra, Paula Coitinho, Cecilia Greif, Gonzalo |
| author_role | author |
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| bitstream.checksumAlgorithm.fl_str_mv | MD5 MD5 MD5 MD5 MD5 |
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| collection | COLIBRI |
| dc.contributor.filiacion.none.fl_str_mv | Hurtado Gutiérrez Joaquín Ignacio, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares. Bentancor María Noel, Comisión Honoraria de Lucha Antituberculosa. Laserra Paula, Comisión Honoraria de Lucha Antituberculosa. Coitinho Cecilia, Comisión Honoraria de Lucha Antituberculosa. Greif Gonzalo, Instituto Pasteur (Montevideo). |
| dc.creator.none.fl_str_mv | Hurtado Gutiérrez, Joaquín Ignacio Bentancor, María Noel Laserra, Paula Coitinho, Cecilia Greif, Gonzalo |
| dc.date.accessioned.none.fl_str_mv | 2025-08-13T17:24:19Z |
| dc.date.available.none.fl_str_mv | 2025-08-13T17:24:19Z |
| dc.date.issued.none.fl_str_mv | 2024 |
| dc.description.abstract.none.fl_txt_mv | This scientific study focuses on tuberculosis (TB) within prison settings, where persons deprived of liberty (PDL) face significantly higher rates of the disease compared to the general population. The research employs the low-cost amplicon sequencing of Mycobacterium tuberculosis strains, aiming first to identify specific lineages and also to detect mutations associated with drug resistance. The method involves multiplex amplification, DNA extraction, and sequencing, providing valuable insights into TB dynamics and resistance-mutation profiles within the prison system at an affordable cost. The study identifies a characteristic lineage (X) circulating among PDLs in the penitentiary system in Uruguay, absent in the general population, and notes its prevalence at prison entry. No high-confidence mutations associated with drug resistance were found. The findings underscore the importance of molecular epidemiology in TB control, emphasizing the potential for intra-prison transmissions and the need for broader studies to understand strain dynamics. |
| dc.description.es.fl_txt_mv | Material suplementario en: www.mdpi.com/article/10.3390/microorganisms12050999/s1 |
| dc.description.sponsorship.none.fl_txt_mv | ANII: FMV_3_2018_1_148367 |
| dc.format.extent.es.fl_str_mv | 8 h |
| dc.format.mimetype.es.fl_str_mv | application/pdf |
| dc.identifier.citation.es.fl_str_mv | Hurtado Gutiérrez, J, Bentancor, M, Laserra, P [y otros autores]. "Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing". Microorganisms. [en línea] 2024, 12(5): 999. 8 h. DOI: 10.3390/ microorganisms12050999 |
| dc.identifier.doi.none.fl_str_mv | 10.3390/ microorganisms12050999 |
| dc.identifier.issn.none.fl_str_mv | 2076-2607 |
| dc.identifier.uri.none.fl_str_mv | https://hdl.handle.net/20.500.12008/51034 |
| dc.language.iso.none.fl_str_mv | en eng |
| dc.publisher.es.fl_str_mv | MDPI |
| dc.relation.none.fl_str_mv | Microorganisms, 2024, 12(5): 999. |
| dc.rights.license.none.fl_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| dc.rights.none.fl_str_mv | info:eu-repo/semantics/openAccess |
| dc.source.none.fl_str_mv | reponame:COLIBRI instname:Universidad de la República instacron:Universidad de la República |
| dc.subject.es.fl_str_mv | Tuberculosis Epidemiology Amplicon sequencing |
| dc.title.none.fl_str_mv | Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing |
| dc.type.es.fl_str_mv | Artículo |
| dc.type.none.fl_str_mv | info:eu-repo/semantics/article |
| dc.type.version.none.fl_str_mv | info:eu-repo/semantics/publishedVersion |
| description | Material suplementario en: www.mdpi.com/article/10.3390/microorganisms12050999/s1 |
| eu_rights_str_mv | openAccess |
| format | article |
| id | COLIBRI_2e4a8064ee809f2712f849d974470cfa |
| identifier_str_mv | Hurtado Gutiérrez, J, Bentancor, M, Laserra, P [y otros autores]. "Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing". Microorganisms. [en línea] 2024, 12(5): 999. 8 h. DOI: 10.3390/ microorganisms12050999 2076-2607 10.3390/ microorganisms12050999 |
| instacron_str | Universidad de la República |
| institution | Universidad de la República |
| instname_str | Universidad de la República |
| language | eng |
| language_invalid_str_mv | en |
| network_acronym_str | COLIBRI |
| network_name_str | COLIBRI |
| oai_identifier_str | oai:colibri.udelar.edu.uy:20.500.12008/51034 |
| publishDate | 2024 |
| reponame_str | COLIBRI |
| repository.mail.fl_str_mv | karina.camps@seciu.edu.uy |
| repository.name.fl_str_mv | COLIBRI - Universidad de la República |
| repository_id_str | 4771 |
| rights_invalid_str_mv | Licencia Creative Commons Atribución (CC - By 4.0) |
| spelling | Hurtado Gutiérrez Joaquín Ignacio, Universidad de la República (Uruguay). Facultad de Ciencias. Centro de Investigaciones Nucleares.Bentancor María Noel, Comisión Honoraria de Lucha Antituberculosa.Laserra Paula, Comisión Honoraria de Lucha Antituberculosa.Coitinho Cecilia, Comisión Honoraria de Lucha Antituberculosa.Greif Gonzalo, Instituto Pasteur (Montevideo).2025-08-13T17:24:19Z2025-08-13T17:24:19Z2024Hurtado Gutiérrez, J, Bentancor, M, Laserra, P [y otros autores]. "Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing". Microorganisms. [en línea] 2024, 12(5): 999. 8 h. DOI: 10.3390/ microorganisms120509992076-2607https://hdl.handle.net/20.500.12008/5103410.3390/ microorganisms12050999Material suplementario en: www.mdpi.com/article/10.3390/microorganisms12050999/s1This scientific study focuses on tuberculosis (TB) within prison settings, where persons deprived of liberty (PDL) face significantly higher rates of the disease compared to the general population. The research employs the low-cost amplicon sequencing of Mycobacterium tuberculosis strains, aiming first to identify specific lineages and also to detect mutations associated with drug resistance. The method involves multiplex amplification, DNA extraction, and sequencing, providing valuable insights into TB dynamics and resistance-mutation profiles within the prison system at an affordable cost. The study identifies a characteristic lineage (X) circulating among PDLs in the penitentiary system in Uruguay, absent in the general population, and notes its prevalence at prison entry. No high-confidence mutations associated with drug resistance were found. The findings underscore the importance of molecular epidemiology in TB control, emphasizing the potential for intra-prison transmissions and the need for broader studies to understand strain dynamics.Submitted by Pintos Natalia (nataliapintosmvd@gmail.com) on 2025-08-08T12:52:49Z No. of bitstreams: 2 license_rdf: 24942 bytes, checksum: 58cb336ce230a47d2f88ad02838a665f (MD5) 10.3390-microorganisms12050999.pdf: 386046 bytes, checksum: aa73cd926a5acec5351ed406485c41b4 (MD5)Approved for entry into archive by Faget Cecilia (lfaget@fcien.edu.uy) on 2025-08-13T14:28:10Z (GMT) No. of bitstreams: 2 license_rdf: 24942 bytes, checksum: 58cb336ce230a47d2f88ad02838a665f (MD5) 10.3390-microorganisms12050999.pdf: 386046 bytes, checksum: aa73cd926a5acec5351ed406485c41b4 (MD5)Made available in DSpace by Luna Fabiana (fabiana.luna@seciu.edu.uy) on 2025-08-13T17:24:19Z (GMT). No. of bitstreams: 2 license_rdf: 24942 bytes, checksum: 58cb336ce230a47d2f88ad02838a665f (MD5) 10.3390-microorganisms12050999.pdf: 386046 bytes, checksum: aa73cd926a5acec5351ed406485c41b4 (MD5) Previous issue date: 2024ANII: FMV_3_2018_1_1483678 happlication/pdfenengMDPIMicroorganisms, 2024, 12(5): 999.Las obras depositadas en el Repositorio se rigen por la Ordenanza de los Derechos de la Propiedad Intelectual de la Universidad de la República.(Res. Nº 91 de C.D.C. de 8/III/1994 – D.O. 7/IV/1994) y por la Ordenanza del Repositorio Abierto de la Universidad de la República (Res. 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públicahttps://udelar.edu.uy/https://www.colibri.udelar.edu.uy/oai/requestkarina.camps@seciu.edu.uyUruguayopendoar:47712025-08-13T17:24:19COLIBRI - Universidad de la Repúblicafalse |
| spellingShingle | Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing Hurtado Gutiérrez, Joaquín Ignacio Tuberculosis Epidemiology Amplicon sequencing |
| status_str | publishedVersion |
| title | Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing |
| title_full | Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing |
| title_fullStr | Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing |
| title_full_unstemmed | Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing |
| title_short | Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing |
| title_sort | Specific Mycobacterium tuberculosis strain circulating in prison revealed by cost-effective amplicon sequencing |
| topic | Tuberculosis Epidemiology Amplicon sequencing |
| url | https://hdl.handle.net/20.500.12008/51034 |
